Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310

Patrick G. Halbur, Prem S. Paul, Eric M. Vaughn, John J. Andrews

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The pathogenicity of porcine respiratory coronavirus (PRCV) isolate AR310 was determined for gnotobiotic pigs. PRCV-AR310 was isolated from the intestines of a nursery pig from a herd with endemic transmissible gastroenteritis. The AR310 isolate was plaque purified and cell culture propagated, passed once in a gnotobiotic pig, then used as inoculum for a gnotobiotic pig pathogenicity study. Eight pigs were inoculated oronasally with 2 × 106 plaque-forming units of PRCV-AR310. Eight pigs served as controls and received cell culture medium. Two pigs from each group were necropsied at 3, 5, 10, and 15 days postinoculation (DPI). There was moderate multifocal to coalescing reddish tan consolidation of 60% of the lung by 10 DPI. Microscopic examination revealed a necrotizing and proliferative bronchointerstitial pneumonia characterized by necrosis, squamous metaplasia, dysplasia, proliferation of airway epithelium, mononuclear cell infiltration of alveolar septa, mild type II pneumocyte proliferation, and lymphohistiocytic alveolar exudation. The microscopic lesions were mild by 3 DPI, moderate by 5 DPI, severe by 10 DPI, and mostly resolved by 15 DPI. No lesions were observed in the intestines of these pigs. There was no clinical respiratory disease. Control pigs remained normal and had no lesions. PRCV was isolated from the lungs but not from the intestines of inoculated pigs. PRCV was not isolated from the lungs or intestines of control pigs. PRCV was also isolated from the nasal and rectal swabs of inoculated but not of control pigs.

Original languageEnglish (US)
Pages (from-to)184-188
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume5
Issue number2
DOIs
StatePublished - Apr 1993

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Porcine Respiratory Coronavirus
Germ-Free Life
Transmissible gastroenteritis virus
pneumonia
Reproduction
Pneumonia
Swine
swine
Intestines
intestines
lesions (animal)
Alveolar Epithelial Cells
lungs
Lung
Virulence
cell culture
pathogenicity
Cell Culture Techniques
transmissible gastroenteritis
pneumocytes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310. / Halbur, Patrick G.; Paul, Prem S.; Vaughn, Eric M.; Andrews, John J.

In: Journal of Veterinary Diagnostic Investigation, Vol. 5, No. 2, 04.1993, p. 184-188.

Research output: Contribution to journalArticle

Halbur, Patrick G. ; Paul, Prem S. ; Vaughn, Eric M. ; Andrews, John J. / Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310. In: Journal of Veterinary Diagnostic Investigation. 1993 ; Vol. 5, No. 2. pp. 184-188.
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