Experimental investigation of needles, syringes and techniques for intravitreal injections

Vinicius S. De Stefano, Jose Jk Abechain, Luiz Fs de Almeida, Diego M. Verginassi, Eduardo B. Rodrigues, Edna Freymuller, Maurício Maia, Octaviano Magalhaes, Quan Dong Nguyen, Michel E. Farah

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: To assess the techniques and materials used in intravitreal injections. Design: Descriptive study realized at the Vision Institute of the Federal University of São Paulo, Brazil. Samples: Different brands of needles and syringes, as well as enucleated porcine eyeballs. Methods: The ultra-structures of commonly used needles were analysed by scanning electron microscope, and they were compared using different criteria, such as irregularities and debris from the lubrication process. The scleral incision was also assessed using needles of different brands and sizes. Accuracies in drug administration were studied by comparing the residual and delivered volume of needles and also by the analysis of reflux after intravitreal injections. Main Outcome Measures: Efficiency and quality of materials used in intravitreal injections. Results: Ultra-structure analyses showed that all needles had different types of irregularities. Some photographs showed debris from the lubrication process, especially in BD needles. Scleral incision analysis showed a tendency of reducing the ocular damage with increasing gauge (P=0.024). The investigation of delivery accuracy showed that almost all needles underdosed the amount injected (P<0.05), and that the reflux could be minimized by tunnelled injections with thinner needles. Conclusion: Needles used in intravitreal injections possess many irregularities in their structures, which may cause different injection outcomes. Analyses of scleral incisions showed that the larger the needle gauge, the lesser the scleral damage and the risk of complications. Moreover, drug administration inaccuracies might be one of the causes for some unsuccessful attempts of treatment.

Original languageEnglish (US)
Pages (from-to)236-242
Number of pages7
JournalClinical and Experimental Ophthalmology
Volume39
Issue number3
DOIs
StatePublished - Apr 1 2011

Fingerprint

Intravitreal Injections
Syringes
Needles
Lubrication
Injections
Residual Volume
Pharmaceutical Preparations
Brazil
Swine
Outcome Assessment (Health Care)
Electrons

Keywords

  • Intravitreal injection
  • Needle
  • Retinal pharmacotherapy
  • Vitreal reflux
  • Vitreoretinal surgery

ASJC Scopus subject areas

  • Ophthalmology

Cite this

De Stefano, V. S., Abechain, J. J., de Almeida, L. F., Verginassi, D. M., Rodrigues, E. B., Freymuller, E., ... Farah, M. E. (2011). Experimental investigation of needles, syringes and techniques for intravitreal injections. Clinical and Experimental Ophthalmology, 39(3), 236-242. https://doi.org/10.1111/j.1442-9071.2010.02447.x

Experimental investigation of needles, syringes and techniques for intravitreal injections. / De Stefano, Vinicius S.; Abechain, Jose Jk; de Almeida, Luiz Fs; Verginassi, Diego M.; Rodrigues, Eduardo B.; Freymuller, Edna; Maia, Maurício; Magalhaes, Octaviano; Nguyen, Quan Dong; Farah, Michel E.

In: Clinical and Experimental Ophthalmology, Vol. 39, No. 3, 01.04.2011, p. 236-242.

Research output: Contribution to journalArticle

De Stefano, VS, Abechain, JJ, de Almeida, LF, Verginassi, DM, Rodrigues, EB, Freymuller, E, Maia, M, Magalhaes, O, Nguyen, QD & Farah, ME 2011, 'Experimental investigation of needles, syringes and techniques for intravitreal injections', Clinical and Experimental Ophthalmology, vol. 39, no. 3, pp. 236-242. https://doi.org/10.1111/j.1442-9071.2010.02447.x
De Stefano VS, Abechain JJ, de Almeida LF, Verginassi DM, Rodrigues EB, Freymuller E et al. Experimental investigation of needles, syringes and techniques for intravitreal injections. Clinical and Experimental Ophthalmology. 2011 Apr 1;39(3):236-242. https://doi.org/10.1111/j.1442-9071.2010.02447.x
De Stefano, Vinicius S. ; Abechain, Jose Jk ; de Almeida, Luiz Fs ; Verginassi, Diego M. ; Rodrigues, Eduardo B. ; Freymuller, Edna ; Maia, Maurício ; Magalhaes, Octaviano ; Nguyen, Quan Dong ; Farah, Michel E. / Experimental investigation of needles, syringes and techniques for intravitreal injections. In: Clinical and Experimental Ophthalmology. 2011 ; Vol. 39, No. 3. pp. 236-242.
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AU - Rodrigues, Eduardo B.

AU - Freymuller, Edna

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