Experimental infection of calves with bovine viral diarrhea virus genotype II (NY-93)

Anselmo C. Odeón, Clayton L Kelling, Donald J. Marshall, E. Sonia Estela, Edward J. Dubovi, Ruben O. Donis

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

To ascertain the virulence of bovine viral diarrhea virus (BVDV) genotype II, isolate NY-93 was inoculated intranasally into 3 calves, 2 of which were treated with a synthetic glucocorticoid prior to and after virus inoculation. Anorexia, fever (up to 42 C), dyspnea, and hemorrhagic diarrhea developed 6 days after intranasal inoculation with BVDV NY-93. The condition of all calves deteriorated further until the end of the study on day 14 postinoculation. The most significant postmortem macroscopic changes in all calves were limited to the gastrointestinal tract and consisted of moderate to severe congestion of the mucosa with multifocal hemorrhages. Microscopic lesions found in the gastrointestinal tract were similar to those observed in mucosal disease, including degeneration and necrosis of crypt epithelium and necrosis of lymphoid tissue throughout the ileum, colon, and rectum. The basal stratum of the epithelium of tongue, esophagus, and rumen had scattered individual necrotic cells. Spleen and lymph nodes had lymphocytolysis and severe lymphoid depletion. Severe acute fibrinous bronchopneumonia was present in dexamethasone-treated calves. Abundant viral antigen was detected by immunohistochemistry in the squamous epithelium of tongue, esophagus, and forestomachs. BVDV antigen was prominent in cells of the media of small arteries and endothelial cells. The presence of infectious virus in tissues correlated with an absence of circulating neutralizing antibodies. These findings highlight the potential of BVDV genotype II to cause severe disease in normal and stressed cattle.

Original languageEnglish (US)
Pages (from-to)221-228
Number of pages8
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number3
DOIs
StatePublished - May 1999

Fingerprint

Bovine Viral Diarrhea Viruses
Bovine viral diarrhea virus
Genotype
calves
epithelium
Epithelium
genotype
esophagus
Infection
tongue
Tongue
infection
Esophagus
gastrointestinal system
Gastrointestinal Tract
necrosis
Necrosis
synthetic glucocorticoids
Postmortem Changes
postmortem changes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Experimental infection of calves with bovine viral diarrhea virus genotype II (NY-93). / Odeón, Anselmo C.; Kelling, Clayton L; Marshall, Donald J.; Estela, E. Sonia; Dubovi, Edward J.; Donis, Ruben O.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 3, 05.1999, p. 221-228.

Research output: Contribution to journalArticle

Odeón, Anselmo C. ; Kelling, Clayton L ; Marshall, Donald J. ; Estela, E. Sonia ; Dubovi, Edward J. ; Donis, Ruben O. / Experimental infection of calves with bovine viral diarrhea virus genotype II (NY-93). In: Journal of Veterinary Diagnostic Investigation. 1999 ; Vol. 11, No. 3. pp. 221-228.
@article{e3fc06c2941e43a4b9ce2d821ac50d76,
title = "Experimental infection of calves with bovine viral diarrhea virus genotype II (NY-93)",
abstract = "To ascertain the virulence of bovine viral diarrhea virus (BVDV) genotype II, isolate NY-93 was inoculated intranasally into 3 calves, 2 of which were treated with a synthetic glucocorticoid prior to and after virus inoculation. Anorexia, fever (up to 42 C), dyspnea, and hemorrhagic diarrhea developed 6 days after intranasal inoculation with BVDV NY-93. The condition of all calves deteriorated further until the end of the study on day 14 postinoculation. The most significant postmortem macroscopic changes in all calves were limited to the gastrointestinal tract and consisted of moderate to severe congestion of the mucosa with multifocal hemorrhages. Microscopic lesions found in the gastrointestinal tract were similar to those observed in mucosal disease, including degeneration and necrosis of crypt epithelium and necrosis of lymphoid tissue throughout the ileum, colon, and rectum. The basal stratum of the epithelium of tongue, esophagus, and rumen had scattered individual necrotic cells. Spleen and lymph nodes had lymphocytolysis and severe lymphoid depletion. Severe acute fibrinous bronchopneumonia was present in dexamethasone-treated calves. Abundant viral antigen was detected by immunohistochemistry in the squamous epithelium of tongue, esophagus, and forestomachs. BVDV antigen was prominent in cells of the media of small arteries and endothelial cells. The presence of infectious virus in tissues correlated with an absence of circulating neutralizing antibodies. These findings highlight the potential of BVDV genotype II to cause severe disease in normal and stressed cattle.",
author = "Ode{\'o}n, {Anselmo C.} and Kelling, {Clayton L} and Marshall, {Donald J.} and Estela, {E. Sonia} and Dubovi, {Edward J.} and Donis, {Ruben O.}",
year = "1999",
month = "5",
doi = "10.1177/104063879901100303",
language = "English (US)",
volume = "11",
pages = "221--228",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "3",

}

TY - JOUR

T1 - Experimental infection of calves with bovine viral diarrhea virus genotype II (NY-93)

AU - Odeón, Anselmo C.

AU - Kelling, Clayton L

AU - Marshall, Donald J.

AU - Estela, E. Sonia

AU - Dubovi, Edward J.

AU - Donis, Ruben O.

PY - 1999/5

Y1 - 1999/5

N2 - To ascertain the virulence of bovine viral diarrhea virus (BVDV) genotype II, isolate NY-93 was inoculated intranasally into 3 calves, 2 of which were treated with a synthetic glucocorticoid prior to and after virus inoculation. Anorexia, fever (up to 42 C), dyspnea, and hemorrhagic diarrhea developed 6 days after intranasal inoculation with BVDV NY-93. The condition of all calves deteriorated further until the end of the study on day 14 postinoculation. The most significant postmortem macroscopic changes in all calves were limited to the gastrointestinal tract and consisted of moderate to severe congestion of the mucosa with multifocal hemorrhages. Microscopic lesions found in the gastrointestinal tract were similar to those observed in mucosal disease, including degeneration and necrosis of crypt epithelium and necrosis of lymphoid tissue throughout the ileum, colon, and rectum. The basal stratum of the epithelium of tongue, esophagus, and rumen had scattered individual necrotic cells. Spleen and lymph nodes had lymphocytolysis and severe lymphoid depletion. Severe acute fibrinous bronchopneumonia was present in dexamethasone-treated calves. Abundant viral antigen was detected by immunohistochemistry in the squamous epithelium of tongue, esophagus, and forestomachs. BVDV antigen was prominent in cells of the media of small arteries and endothelial cells. The presence of infectious virus in tissues correlated with an absence of circulating neutralizing antibodies. These findings highlight the potential of BVDV genotype II to cause severe disease in normal and stressed cattle.

AB - To ascertain the virulence of bovine viral diarrhea virus (BVDV) genotype II, isolate NY-93 was inoculated intranasally into 3 calves, 2 of which were treated with a synthetic glucocorticoid prior to and after virus inoculation. Anorexia, fever (up to 42 C), dyspnea, and hemorrhagic diarrhea developed 6 days after intranasal inoculation with BVDV NY-93. The condition of all calves deteriorated further until the end of the study on day 14 postinoculation. The most significant postmortem macroscopic changes in all calves were limited to the gastrointestinal tract and consisted of moderate to severe congestion of the mucosa with multifocal hemorrhages. Microscopic lesions found in the gastrointestinal tract were similar to those observed in mucosal disease, including degeneration and necrosis of crypt epithelium and necrosis of lymphoid tissue throughout the ileum, colon, and rectum. The basal stratum of the epithelium of tongue, esophagus, and rumen had scattered individual necrotic cells. Spleen and lymph nodes had lymphocytolysis and severe lymphoid depletion. Severe acute fibrinous bronchopneumonia was present in dexamethasone-treated calves. Abundant viral antigen was detected by immunohistochemistry in the squamous epithelium of tongue, esophagus, and forestomachs. BVDV antigen was prominent in cells of the media of small arteries and endothelial cells. The presence of infectious virus in tissues correlated with an absence of circulating neutralizing antibodies. These findings highlight the potential of BVDV genotype II to cause severe disease in normal and stressed cattle.

UR - http://www.scopus.com/inward/record.url?scp=0033129387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033129387&partnerID=8YFLogxK

U2 - 10.1177/104063879901100303

DO - 10.1177/104063879901100303

M3 - Article

C2 - 10353352

AN - SCOPUS:0033129387

VL - 11

SP - 221

EP - 228

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 3

ER -