Experimental evaluation of host adaptation of Lactobacillus reuteri to different vertebrate species

Rebbeca M. Duar, Steven A. Frese, Xiaoxi B. Lin, Samodha C Fernando, Thomas E Burkey, Guergana Tasseva, Daniel A. Peterson, Jochen Blom, Cory Q. Wenzel, Christine M. Szymanski, Jens Walter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The species Lactobacillus reuteri has diversified into host-specific lineages, implying a long-term association with different vertebrates. Strains from rodent lineages show specific adaptations to mice, but the processes underlying the evolution of L. reuteri in other hosts remain unknown. We administered three standardized inocula composed of strains from different host-confined lineages to mice, pigs, chickens, and humans. The ecological performance of each strain in the gastrointestinal tract of each host was determined by typing random colonies recovered from fecal samples collected over five consecutive days postadministration. Results revealed that rodent strains were predominant in mice, confirming previous findings of host adaptation. In chickens, poultry strains of the lineage VI (poultry VI) and human isolates from the same lineage (human VI) were recovered at the highest and second highest rates, respectively. Interestingly, human VI strains were virtually undetected in human feces. These findings, together with ancestral state reconstructions, indicate poultry VI and human VI strains share an evolutionary history with chickens. Genomic analysis revealed that poultry VI strains possess a large and variable accessory genome, whereas human VI strains display low genetic diversity and possess genes encoding antibiotic resistance and capsular polysaccharide synthesis, which might have allowed temporal colonization of humans. Experiments in pigs and humans did not provide evidence of host adaptation of L. reuteri to these hosts. Overall, our findings demonstrate host adaptation of L. reuteri to rodents and chickens, supporting a joint evolution of this bacterial species with several vertebrate hosts, although questions remain about its natural history in humans and pigs.

Original languageEnglish (US)
Article numbere00132-17
JournalApplied and Environmental Microbiology
Volume83
Issue number12
DOIs
StatePublished - Jun 1 2017

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Lactobacillus reuteri
Vertebrates
vertebrate
vertebrates
Chickens
Poultry
poultry
pig
rodent
Rodentia
Swine
chickens
rodents
swine
mice
evaluation
antibiotic resistance
Human Genome
Microbial Drug Resistance
history

Keywords

  • Host adaptation
  • Lactobacillus reuteri
  • Probiotics
  • Symbiosis

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Experimental evaluation of host adaptation of Lactobacillus reuteri to different vertebrate species. / Duar, Rebbeca M.; Frese, Steven A.; Lin, Xiaoxi B.; Fernando, Samodha C; Burkey, Thomas E; Tasseva, Guergana; Peterson, Daniel A.; Blom, Jochen; Wenzel, Cory Q.; Szymanski, Christine M.; Walter, Jens.

In: Applied and Environmental Microbiology, Vol. 83, No. 12, e00132-17, 01.06.2017.

Research output: Contribution to journalArticle

Duar, RM, Frese, SA, Lin, XB, Fernando, SC, Burkey, TE, Tasseva, G, Peterson, DA, Blom, J, Wenzel, CQ, Szymanski, CM & Walter, J 2017, 'Experimental evaluation of host adaptation of Lactobacillus reuteri to different vertebrate species', Applied and Environmental Microbiology, vol. 83, no. 12, e00132-17. https://doi.org/10.1128/AEM.00132-17
Duar, Rebbeca M. ; Frese, Steven A. ; Lin, Xiaoxi B. ; Fernando, Samodha C ; Burkey, Thomas E ; Tasseva, Guergana ; Peterson, Daniel A. ; Blom, Jochen ; Wenzel, Cory Q. ; Szymanski, Christine M. ; Walter, Jens. / Experimental evaluation of host adaptation of Lactobacillus reuteri to different vertebrate species. In: Applied and Environmental Microbiology. 2017 ; Vol. 83, No. 12.
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