Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats

N. P. de Souza, AP Ferragut Cardoso, L. M.M. Gomide, T. R.R. Lima, H. A. Miot, A. J. Martino-Andrade, L. L. Arnold, K. L. Pennington, S. M. Cohen, J. L.V. de Camargo, M. G. Nascimento e Pontes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cryptorchidism (CPT), the most common male congenital abnormality, is variably associated with other male reproductive tract problems. We evaluated if cryptorchid rats develop enhanced testicular susceptibility to dibutyl phthalate (DBP) or acrylamide (AA) after extended exposure. Three studies with rats were performed: (1) in utero and postnatal exposure to DBP or AA; (2) establishment of CPT and orchiopexy; and (3) in utero and postnatal exposures to DBP or AA associated with CPT/orchiopexy. Seminiferous tubules were histologically scored according to the severity of lesions: (1) Rats exposed to DBP (score 1.5) or AA (score 1.1) presented mostly preserved spermatogenesis. Some seminiferous tubules showed vacuolated germinative epithelium, germ cell apoptosis, and a Sertoli cell-only (SCO) pattern. (2) CPT (score 3.3) resulted in decreased absolute testes weights, degenerated and SCO tubules, and spermatogenesis arrest that were reversed by orchiopexy (score 1.1). (3) Exposure to DBP or AA with CPT/orchiopexy led to atrophic testes, spermatogenesis arrest, germ cell exfoliation/multinucleation, and SCO tubules (both chemicals score 2.5). Exposure to chemicals such as DBP or AA prevented the recovery of cryptorchid testes by orchiopexy. The possible role of environmental contaminants should be considered when looking for factors that modulate human testicular disorders associated with CPT.

Original languageEnglish (US)
Pages (from-to)899-913
Number of pages15
JournalHuman and Experimental Toxicology
Volume38
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Dibutyl Phthalate
Cryptorchidism
Acrylamide
Orchiopexy
Sprague Dawley Rats
Rats
Sertoli Cells
Testis
Seminiferous Tubules
Germ Cells
Cells
Spermatogenesis
Epithelium
Impurities
Apoptosis
Weights and Measures
Recovery

Keywords

  • Cryptorchidism
  • acrylamide
  • dibutyl phthalate
  • histology
  • testicular germ cells

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

de Souza, N. P., Cardoso, AP. F., Gomide, L. M. M., Lima, T. R. R., Miot, H. A., Martino-Andrade, A. J., ... Nascimento e Pontes, M. G. (2019). Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats. Human and Experimental Toxicology, 38(8), 899-913. https://doi.org/10.1177/0960327119845040

Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats. / de Souza, N. P.; Cardoso, AP Ferragut; Gomide, L. M.M.; Lima, T. R.R.; Miot, H. A.; Martino-Andrade, A. J.; Arnold, L. L.; Pennington, K. L.; Cohen, S. M.; de Camargo, J. L.V.; Nascimento e Pontes, M. G.

In: Human and Experimental Toxicology, Vol. 38, No. 8, 01.08.2019, p. 899-913.

Research output: Contribution to journalArticle

de Souza, NP, Cardoso, APF, Gomide, LMM, Lima, TRR, Miot, HA, Martino-Andrade, AJ, Arnold, LL, Pennington, KL, Cohen, SM, de Camargo, JLV & Nascimento e Pontes, MG 2019, 'Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats', Human and Experimental Toxicology, vol. 38, no. 8, pp. 899-913. https://doi.org/10.1177/0960327119845040
de Souza, N. P. ; Cardoso, AP Ferragut ; Gomide, L. M.M. ; Lima, T. R.R. ; Miot, H. A. ; Martino-Andrade, A. J. ; Arnold, L. L. ; Pennington, K. L. ; Cohen, S. M. ; de Camargo, J. L.V. ; Nascimento e Pontes, M. G. / Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats. In: Human and Experimental Toxicology. 2019 ; Vol. 38, No. 8. pp. 899-913.
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AU - Miot, H. A.

AU - Martino-Andrade, A. J.

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