Experimental and modeling studies of diffusion in immobilized cell systems

A review of recent literature and patents

Mark Riley, Fernando J. Muzzio, Sebastian C. Reyes

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

In summary, the overall behavior of immobilized cells is controlled by the interplay of nutrient and product diffusion and reaction, cell metabolism, cell proliferation, and cell death. These process are intrinsically linked, and evaluation of one process independent of other effects can be difficult. Substantial overlap exists between much of the experimental and computational work done in this field over the past 10 yr but with little cross communication. We hope that the areas of research described here will provide a basis for communication between researchers in disparate fields working on similar problems. The past several years have yielded significant improvements in the development and operation of immobilized cell systems. Possible progress in the near future may develop through the use of new techniques and approaches developed in other research areas. Models and simulations will continue to increase in complexity in proportion to improvements in computer speed, memory, and cost. An increase in simulation sophistication should focus on developing a more thorough understanding of the effect of the accumulation of wastes, deprivation of nutrients, and increased local concentrations of cellular growth factors. Through a combination of experimental and modeling studies, one can thoroughly evaluate the effects of alterations in multiple parameters on the overall productivity of immobilized cell biocatalysts. Possible experimental progress that may impact future use of immobilized cells includes the development of novel polymeric supports that can respond to external stimuli (electrochemical, pH, salt changes); the use of noninvasive monitoring techniques (such as IR microspectroscopy) to directly characterize molecular transport, cellular metabolism, and cell proliferation in situ; and the development of improved methods to deliver precise amounts of nutrients and oxygen, possibly through the use of controlled release methods or the use of techniques to increase substantially the oxygen tension in the culture medium since oxygen is predominantly the most frequent limiting factor.

Original languageEnglish (US)
Pages (from-to)151-188
Number of pages38
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume80
Issue number2
DOIs
StatePublished - Aug 5 1999

Fingerprint

Immobilized Cells
Patents
Cells
Nutrients
Cell Proliferation
Cell proliferation
Oxygen
Metabolism
Food
Communication
Biocatalysts
Cell death
Research
Culture Media
Intercellular Signaling Peptides and Proteins
Cell Death
Salts
Productivity
Research Personnel
Efficiency

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Experimental and modeling studies of diffusion in immobilized cell systems : A review of recent literature and patents. / Riley, Mark; Muzzio, Fernando J.; Reyes, Sebastian C.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 80, No. 2, 05.08.1999, p. 151-188.

Research output: Contribution to journalReview article

@article{480e68dd79604349a9fb33e6f161e3f0,
title = "Experimental and modeling studies of diffusion in immobilized cell systems: A review of recent literature and patents",
abstract = "In summary, the overall behavior of immobilized cells is controlled by the interplay of nutrient and product diffusion and reaction, cell metabolism, cell proliferation, and cell death. These process are intrinsically linked, and evaluation of one process independent of other effects can be difficult. Substantial overlap exists between much of the experimental and computational work done in this field over the past 10 yr but with little cross communication. We hope that the areas of research described here will provide a basis for communication between researchers in disparate fields working on similar problems. The past several years have yielded significant improvements in the development and operation of immobilized cell systems. Possible progress in the near future may develop through the use of new techniques and approaches developed in other research areas. Models and simulations will continue to increase in complexity in proportion to improvements in computer speed, memory, and cost. An increase in simulation sophistication should focus on developing a more thorough understanding of the effect of the accumulation of wastes, deprivation of nutrients, and increased local concentrations of cellular growth factors. Through a combination of experimental and modeling studies, one can thoroughly evaluate the effects of alterations in multiple parameters on the overall productivity of immobilized cell biocatalysts. Possible experimental progress that may impact future use of immobilized cells includes the development of novel polymeric supports that can respond to external stimuli (electrochemical, pH, salt changes); the use of noninvasive monitoring techniques (such as IR microspectroscopy) to directly characterize molecular transport, cellular metabolism, and cell proliferation in situ; and the development of improved methods to deliver precise amounts of nutrients and oxygen, possibly through the use of controlled release methods or the use of techniques to increase substantially the oxygen tension in the culture medium since oxygen is predominantly the most frequent limiting factor.",
author = "Mark Riley and Muzzio, {Fernando J.} and Reyes, {Sebastian C.}",
year = "1999",
month = "8",
day = "5",
doi = "10.1385/ABAB:80:2:151",
language = "English (US)",
volume = "80",
pages = "151--188",
journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "2",

}

TY - JOUR

T1 - Experimental and modeling studies of diffusion in immobilized cell systems

T2 - A review of recent literature and patents

AU - Riley, Mark

AU - Muzzio, Fernando J.

AU - Reyes, Sebastian C.

PY - 1999/8/5

Y1 - 1999/8/5

N2 - In summary, the overall behavior of immobilized cells is controlled by the interplay of nutrient and product diffusion and reaction, cell metabolism, cell proliferation, and cell death. These process are intrinsically linked, and evaluation of one process independent of other effects can be difficult. Substantial overlap exists between much of the experimental and computational work done in this field over the past 10 yr but with little cross communication. We hope that the areas of research described here will provide a basis for communication between researchers in disparate fields working on similar problems. The past several years have yielded significant improvements in the development and operation of immobilized cell systems. Possible progress in the near future may develop through the use of new techniques and approaches developed in other research areas. Models and simulations will continue to increase in complexity in proportion to improvements in computer speed, memory, and cost. An increase in simulation sophistication should focus on developing a more thorough understanding of the effect of the accumulation of wastes, deprivation of nutrients, and increased local concentrations of cellular growth factors. Through a combination of experimental and modeling studies, one can thoroughly evaluate the effects of alterations in multiple parameters on the overall productivity of immobilized cell biocatalysts. Possible experimental progress that may impact future use of immobilized cells includes the development of novel polymeric supports that can respond to external stimuli (electrochemical, pH, salt changes); the use of noninvasive monitoring techniques (such as IR microspectroscopy) to directly characterize molecular transport, cellular metabolism, and cell proliferation in situ; and the development of improved methods to deliver precise amounts of nutrients and oxygen, possibly through the use of controlled release methods or the use of techniques to increase substantially the oxygen tension in the culture medium since oxygen is predominantly the most frequent limiting factor.

AB - In summary, the overall behavior of immobilized cells is controlled by the interplay of nutrient and product diffusion and reaction, cell metabolism, cell proliferation, and cell death. These process are intrinsically linked, and evaluation of one process independent of other effects can be difficult. Substantial overlap exists between much of the experimental and computational work done in this field over the past 10 yr but with little cross communication. We hope that the areas of research described here will provide a basis for communication between researchers in disparate fields working on similar problems. The past several years have yielded significant improvements in the development and operation of immobilized cell systems. Possible progress in the near future may develop through the use of new techniques and approaches developed in other research areas. Models and simulations will continue to increase in complexity in proportion to improvements in computer speed, memory, and cost. An increase in simulation sophistication should focus on developing a more thorough understanding of the effect of the accumulation of wastes, deprivation of nutrients, and increased local concentrations of cellular growth factors. Through a combination of experimental and modeling studies, one can thoroughly evaluate the effects of alterations in multiple parameters on the overall productivity of immobilized cell biocatalysts. Possible experimental progress that may impact future use of immobilized cells includes the development of novel polymeric supports that can respond to external stimuli (electrochemical, pH, salt changes); the use of noninvasive monitoring techniques (such as IR microspectroscopy) to directly characterize molecular transport, cellular metabolism, and cell proliferation in situ; and the development of improved methods to deliver precise amounts of nutrients and oxygen, possibly through the use of controlled release methods or the use of techniques to increase substantially the oxygen tension in the culture medium since oxygen is predominantly the most frequent limiting factor.

UR - http://www.scopus.com/inward/record.url?scp=0032782696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032782696&partnerID=8YFLogxK

U2 - 10.1385/ABAB:80:2:151

DO - 10.1385/ABAB:80:2:151

M3 - Review article

VL - 80

SP - 151

EP - 188

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

SN - 0273-2289

IS - 2

ER -