Abstract
Electrospun nanofibers have shown great potential as scaffolds for regenerative medicine because of its biomimicry. However, traditional two-dimensional electrospun nanofiber mats inhibit their applications because of the dense structure and lack of effective cell infiltration. Herein, we report a new method of expanding electrospun nanofiber mats in the third dimension using a modified gas-foaming technique. The resulting nanofiber scaffolds show layered structures with controllable gap widths and layer thicknesses on the order of microns. Expanded nanofiber scaffolds possess significantly higher porosity than traditional two-dimensional nanofiber membranes, while simultaneously maintaining nanotopographic cues. The distributions of gap widths and layer thicknesses are directly dependent on the processing time of nanofiber mats within the gas bubble forming solution. In vitro testing demonstrates robust cellular infiltration and proliferation within expanded nanofiber scaffolds as compared to limited cellular proliferation on the surface of traditional nanofiber mats. Importantly, cell alignment was observed throughout the expanded and aligned nanofiber scaffolds after incubation for 7 days. The presented method was further applied to fabricate tubular scaffolds composed of expanded nanofibers. Together, this novel class of scaffolds holds significant promise for applications in regenerative medicine and building 3D in vitro tissue models for drug screening and biological study.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 991-1001 |
| Number of pages | 11 |
| Journal | ACS Biomaterials Science and Engineering |
| Volume | 1 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 12 2015 |
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Keywords
- cell infiltration
- gas bubbles
- nanofiber scaffolds
- regenerative medicine
- three-dimensional
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Expanding Two-Dimensional Electrospun Nanofiber Membranes in the Third Dimension by a Modified Gas-Foaming Technique. / Jiang, Jiang; Carlson, Mark Alan; Teusink, Matthew J; Wang, Hongjun; MacEwan, Matthew R.; Xie, Jingwei.
In: ACS Biomaterials Science and Engineering, Vol. 1, No. 10, 12.10.2015, p. 991-1001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expanding Two-Dimensional Electrospun Nanofiber Membranes in the Third Dimension by a Modified Gas-Foaming Technique
AU - Jiang, Jiang
AU - Carlson, Mark Alan
AU - Teusink, Matthew J
AU - Wang, Hongjun
AU - MacEwan, Matthew R.
AU - Xie, Jingwei
PY - 2015/10/12
Y1 - 2015/10/12
N2 - Electrospun nanofibers have shown great potential as scaffolds for regenerative medicine because of its biomimicry. However, traditional two-dimensional electrospun nanofiber mats inhibit their applications because of the dense structure and lack of effective cell infiltration. Herein, we report a new method of expanding electrospun nanofiber mats in the third dimension using a modified gas-foaming technique. The resulting nanofiber scaffolds show layered structures with controllable gap widths and layer thicknesses on the order of microns. Expanded nanofiber scaffolds possess significantly higher porosity than traditional two-dimensional nanofiber membranes, while simultaneously maintaining nanotopographic cues. The distributions of gap widths and layer thicknesses are directly dependent on the processing time of nanofiber mats within the gas bubble forming solution. In vitro testing demonstrates robust cellular infiltration and proliferation within expanded nanofiber scaffolds as compared to limited cellular proliferation on the surface of traditional nanofiber mats. Importantly, cell alignment was observed throughout the expanded and aligned nanofiber scaffolds after incubation for 7 days. The presented method was further applied to fabricate tubular scaffolds composed of expanded nanofibers. Together, this novel class of scaffolds holds significant promise for applications in regenerative medicine and building 3D in vitro tissue models for drug screening and biological study.
AB - Electrospun nanofibers have shown great potential as scaffolds for regenerative medicine because of its biomimicry. However, traditional two-dimensional electrospun nanofiber mats inhibit their applications because of the dense structure and lack of effective cell infiltration. Herein, we report a new method of expanding electrospun nanofiber mats in the third dimension using a modified gas-foaming technique. The resulting nanofiber scaffolds show layered structures with controllable gap widths and layer thicknesses on the order of microns. Expanded nanofiber scaffolds possess significantly higher porosity than traditional two-dimensional nanofiber membranes, while simultaneously maintaining nanotopographic cues. The distributions of gap widths and layer thicknesses are directly dependent on the processing time of nanofiber mats within the gas bubble forming solution. In vitro testing demonstrates robust cellular infiltration and proliferation within expanded nanofiber scaffolds as compared to limited cellular proliferation on the surface of traditional nanofiber mats. Importantly, cell alignment was observed throughout the expanded and aligned nanofiber scaffolds after incubation for 7 days. The presented method was further applied to fabricate tubular scaffolds composed of expanded nanofibers. Together, this novel class of scaffolds holds significant promise for applications in regenerative medicine and building 3D in vitro tissue models for drug screening and biological study.
KW - cell infiltration
KW - gas bubbles
KW - nanofiber scaffolds
KW - regenerative medicine
KW - three-dimensional
UR - http://www.scopus.com/inward/record.url?scp=84991523402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991523402&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.5b00238
DO - 10.1021/acsbiomaterials.5b00238
M3 - Article
AN - SCOPUS:84991523402
VL - 1
SP - 991
EP - 1001
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 10
ER -