Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches

Rebekah L. Gundry, Irina Tchernyshyov, Shijun Sheng, Yelena Tarasova, Kimberly Raginski, Kenneth R. Boheler, Jennifer E. Van Eyk

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.

Original languageEnglish (US)
Pages (from-to)2728-2732
Number of pages5
JournalProteomics
Volume10
Issue number14
DOIs
StatePublished - Jul 1 2010

Fingerprint

Proteome
Stem cells
Proteomics
Protein Isoforms
Proteins
Cell Line
Digestion
Cells
Peptides
Mouse Embryonic Stem Cells

Keywords

  • 1-D gel electrophoresis
  • 2-D chromatography
  • Cell biology
  • Embryonic stem cell
  • Isoforms
  • Shotgun proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Gundry, R. L., Tchernyshyov, I., Sheng, S., Tarasova, Y., Raginski, K., Boheler, K. R., & Van Eyk, J. E. (2010). Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches. Proteomics, 10(14), 2728-2732. https://doi.org/10.1002/pmic.201000039

Expanding the mouse embryonic stem cell proteome : Combining three proteomic approaches. / Gundry, Rebekah L.; Tchernyshyov, Irina; Sheng, Shijun; Tarasova, Yelena; Raginski, Kimberly; Boheler, Kenneth R.; Van Eyk, Jennifer E.

In: Proteomics, Vol. 10, No. 14, 01.07.2010, p. 2728-2732.

Research output: Contribution to journalArticle

Gundry, RL, Tchernyshyov, I, Sheng, S, Tarasova, Y, Raginski, K, Boheler, KR & Van Eyk, JE 2010, 'Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches', Proteomics, vol. 10, no. 14, pp. 2728-2732. https://doi.org/10.1002/pmic.201000039
Gundry RL, Tchernyshyov I, Sheng S, Tarasova Y, Raginski K, Boheler KR et al. Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches. Proteomics. 2010 Jul 1;10(14):2728-2732. https://doi.org/10.1002/pmic.201000039
Gundry, Rebekah L. ; Tchernyshyov, Irina ; Sheng, Shijun ; Tarasova, Yelena ; Raginski, Kimberly ; Boheler, Kenneth R. ; Van Eyk, Jennifer E. / Expanding the mouse embryonic stem cell proteome : Combining three proteomic approaches. In: Proteomics. 2010 ; Vol. 10, No. 14. pp. 2728-2732.
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