Exercise training improves the defective centrally mediated erectile responses in rats with type I diabetes

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Abstract

Introduction. Erectile dysfunction is a serious and common complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for the penile erection. Aim. The goal of the present study was to determine the impact of exercise training (ExT) on the centrally mediated erectile dysfunction in streptozotocin (STZ)-induced type I diabetic (T1D) rats. Methods. Male Sprague-Dawley rats were injected with STZ to induce diabetes mellitus. Three weeks after STZ or vehicle injections, rats were assigned to either ExT (treadmill running for 3-4 weeks) or sedentary groups to produce four experimental groups: control+sedentary, T1D+sedentary, control+ExT, and T1D+ExT. Main Outcome Measure. After 3-4 weeks ExT, central N-methyl-D-aspartic acid (NMDA) or sodium nitroprusside (SNP)-induced penile erectile responses were measured. Neuronal nitric oxide synthase (nNOS) expression in the paraventricular nucleus (PVN) of the hypothalamus was measured by using histochemistry, real time polymerase chain reaction (PCR) and Western blot approaches. Results. In rats with T1D, ExT significantly improved the blunted erectile response, and the intracavernous pressure changes to NMDA (50ng) microinjection within the PVN (T1D+ExT: 3.0±0.6 penile erection/rat; T1D+sedentary: 0.5±0.3 penile erection/rat within 20 minutes, P<0.05). ExT improved erectile dysfunction induced by central administration of exogenous nitric oxide (NO) donor, SNP in T1D rats. Other behavior responses including yawning and stretching, induced by central NMDA and SNP microinjection were also significantly increased in T1D rats after ExT. Furthermore, we found that ExT restored the nNOS mRNA and protein expression in the PVN in T1D rats. Conclusions. These results suggest that ExT may have beneficial effects on the erectile dysfunction in diabetes through improvement of NO bioavailability within the PVN. Thus, ExT may be used as therapeutic modality to up-regulate nNOS within the PVN and improve the central component of the erectile dysfunction in diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)3086-3097
Number of pages12
JournalJournal of Sexual Medicine
Volume8
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Type 1 Diabetes Mellitus
Exercise
Paraventricular Hypothalamic Nucleus
Erectile Dysfunction
Penile Erection
Nitric Oxide Synthase Type I
Nitroprusside
N-Methylaspartate
Streptozocin
Microinjections
Diabetes Mellitus
Yawning
Nitric Oxide Donors
Diabetes Complications
Running
Biological Availability
Hypothalamus
Sprague Dawley Rats
Real-Time Polymerase Chain Reaction
Nitric Oxide

Keywords

  • Central mechanisms of penile erection
  • Central nervous system
  • Exercise training
  • Type I diabetes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Urology

Cite this

@article{d6d956a1e23e4e08801e087da1454c77,
title = "Exercise training improves the defective centrally mediated erectile responses in rats with type I diabetes",
abstract = "Introduction. Erectile dysfunction is a serious and common complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for the penile erection. Aim. The goal of the present study was to determine the impact of exercise training (ExT) on the centrally mediated erectile dysfunction in streptozotocin (STZ)-induced type I diabetic (T1D) rats. Methods. Male Sprague-Dawley rats were injected with STZ to induce diabetes mellitus. Three weeks after STZ or vehicle injections, rats were assigned to either ExT (treadmill running for 3-4 weeks) or sedentary groups to produce four experimental groups: control+sedentary, T1D+sedentary, control+ExT, and T1D+ExT. Main Outcome Measure. After 3-4 weeks ExT, central N-methyl-D-aspartic acid (NMDA) or sodium nitroprusside (SNP)-induced penile erectile responses were measured. Neuronal nitric oxide synthase (nNOS) expression in the paraventricular nucleus (PVN) of the hypothalamus was measured by using histochemistry, real time polymerase chain reaction (PCR) and Western blot approaches. Results. In rats with T1D, ExT significantly improved the blunted erectile response, and the intracavernous pressure changes to NMDA (50ng) microinjection within the PVN (T1D+ExT: 3.0±0.6 penile erection/rat; T1D+sedentary: 0.5±0.3 penile erection/rat within 20 minutes, P<0.05). ExT improved erectile dysfunction induced by central administration of exogenous nitric oxide (NO) donor, SNP in T1D rats. Other behavior responses including yawning and stretching, induced by central NMDA and SNP microinjection were also significantly increased in T1D rats after ExT. Furthermore, we found that ExT restored the nNOS mRNA and protein expression in the PVN in T1D rats. Conclusions. These results suggest that ExT may have beneficial effects on the erectile dysfunction in diabetes through improvement of NO bioavailability within the PVN. Thus, ExT may be used as therapeutic modality to up-regulate nNOS within the PVN and improve the central component of the erectile dysfunction in diabetes mellitus.",
keywords = "Central mechanisms of penile erection, Central nervous system, Exercise training, Type I diabetes",
author = "Hong Zheng and Mayhan, {William G.} and Patel, {Kaushik P.}",
year = "2011",
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language = "English (US)",
volume = "8",
pages = "3086--3097",
journal = "Journal of Sexual Medicine",
issn = "1743-6095",
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T1 - Exercise training improves the defective centrally mediated erectile responses in rats with type I diabetes

AU - Zheng, Hong

AU - Mayhan, William G.

AU - Patel, Kaushik P.

PY - 2011/11

Y1 - 2011/11

N2 - Introduction. Erectile dysfunction is a serious and common complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for the penile erection. Aim. The goal of the present study was to determine the impact of exercise training (ExT) on the centrally mediated erectile dysfunction in streptozotocin (STZ)-induced type I diabetic (T1D) rats. Methods. Male Sprague-Dawley rats were injected with STZ to induce diabetes mellitus. Three weeks after STZ or vehicle injections, rats were assigned to either ExT (treadmill running for 3-4 weeks) or sedentary groups to produce four experimental groups: control+sedentary, T1D+sedentary, control+ExT, and T1D+ExT. Main Outcome Measure. After 3-4 weeks ExT, central N-methyl-D-aspartic acid (NMDA) or sodium nitroprusside (SNP)-induced penile erectile responses were measured. Neuronal nitric oxide synthase (nNOS) expression in the paraventricular nucleus (PVN) of the hypothalamus was measured by using histochemistry, real time polymerase chain reaction (PCR) and Western blot approaches. Results. In rats with T1D, ExT significantly improved the blunted erectile response, and the intracavernous pressure changes to NMDA (50ng) microinjection within the PVN (T1D+ExT: 3.0±0.6 penile erection/rat; T1D+sedentary: 0.5±0.3 penile erection/rat within 20 minutes, P<0.05). ExT improved erectile dysfunction induced by central administration of exogenous nitric oxide (NO) donor, SNP in T1D rats. Other behavior responses including yawning and stretching, induced by central NMDA and SNP microinjection were also significantly increased in T1D rats after ExT. Furthermore, we found that ExT restored the nNOS mRNA and protein expression in the PVN in T1D rats. Conclusions. These results suggest that ExT may have beneficial effects on the erectile dysfunction in diabetes through improvement of NO bioavailability within the PVN. Thus, ExT may be used as therapeutic modality to up-regulate nNOS within the PVN and improve the central component of the erectile dysfunction in diabetes mellitus.

AB - Introduction. Erectile dysfunction is a serious and common complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for the penile erection. Aim. The goal of the present study was to determine the impact of exercise training (ExT) on the centrally mediated erectile dysfunction in streptozotocin (STZ)-induced type I diabetic (T1D) rats. Methods. Male Sprague-Dawley rats were injected with STZ to induce diabetes mellitus. Three weeks after STZ or vehicle injections, rats were assigned to either ExT (treadmill running for 3-4 weeks) or sedentary groups to produce four experimental groups: control+sedentary, T1D+sedentary, control+ExT, and T1D+ExT. Main Outcome Measure. After 3-4 weeks ExT, central N-methyl-D-aspartic acid (NMDA) or sodium nitroprusside (SNP)-induced penile erectile responses were measured. Neuronal nitric oxide synthase (nNOS) expression in the paraventricular nucleus (PVN) of the hypothalamus was measured by using histochemistry, real time polymerase chain reaction (PCR) and Western blot approaches. Results. In rats with T1D, ExT significantly improved the blunted erectile response, and the intracavernous pressure changes to NMDA (50ng) microinjection within the PVN (T1D+ExT: 3.0±0.6 penile erection/rat; T1D+sedentary: 0.5±0.3 penile erection/rat within 20 minutes, P<0.05). ExT improved erectile dysfunction induced by central administration of exogenous nitric oxide (NO) donor, SNP in T1D rats. Other behavior responses including yawning and stretching, induced by central NMDA and SNP microinjection were also significantly increased in T1D rats after ExT. Furthermore, we found that ExT restored the nNOS mRNA and protein expression in the PVN in T1D rats. Conclusions. These results suggest that ExT may have beneficial effects on the erectile dysfunction in diabetes through improvement of NO bioavailability within the PVN. Thus, ExT may be used as therapeutic modality to up-regulate nNOS within the PVN and improve the central component of the erectile dysfunction in diabetes mellitus.

KW - Central mechanisms of penile erection

KW - Central nervous system

KW - Exercise training

KW - Type I diabetes

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