Exercise-induced oxidative stress: The effects of β-alanine supplementation in women

A. E. Smith, J. R. Stout, K. L. Kendall, D. H. Fukuda, J. T. Cramer

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the effects of β-alanine supplementation on markers of oxidative stress. Twenty-four women (age: 21.7 ± 2.1 years; VO2max: 2.6 ± 0.3 l min-1) were randomly assigned, in a double-blind fashion, to a β-alanine (BA, 2 × 800 mg tablets, 3× daily; CarnoSyn®; n = 13) or placebo (PL, 2 × 800 mg maltodextrin tablets, 3× daily; n = 11) group. A graded oxygen consumption test (VO2max) was performed to evaluate VO2max, time to exhaustion, ventilatory threshold and establish peak velocity (PV). A 40-min treadmill run was used to induce oxidative stress. Total antioxidant capacity, superoxide dismutase, 8-isoprostane (8ISO) and reduced glutathione were measured. Heart rate and ratings of perceived exertion were recorded during the 40 min run. Separate three- [4 × 2 × 2; acute (base vs. IP vs. 2 vs. 4 h) × chronic (pre- vs. post-) × treatment (BA vs. PL)] and two- [2 × 2; time (pre-supplement vs. post-supplement) × treatment (BA vs. PL)] way ANOVAs were used for analyses. There was a significant increase in VO2max (p = 0.009), independent of treatment, with no significant changes in TTE (p = 0.074) or VT (p = 0.344). Ratings of perceived exertion values were significantly improved from pre- to post-supplementation for the BA group only at 40 min (p = 0.02). The ANOVA model demonstrated no significant treatment effects on oxidative stress. The chronic effects of BA supplementation demonstrated little antioxidant potential, in women, and little influence on aerobic performance assessments.

Original languageEnglish (US)
Pages (from-to)77-90
Number of pages14
JournalAmino Acids
Volume43
Issue number1
DOIs
StatePublished - Jul 1 2012

Fingerprint

Oxidative stress
Alanine
8-epi-prostaglandin F2alpha
Oxidative Stress
Exercise
Analysis of variance (ANOVA)
Tablets
Antioxidants
Exercise equipment
Analysis of Variance
Superoxide Dismutase
Glutathione
Therapeutics
Oxygen Consumption
Oxygen
Heart Rate
Placebos

Keywords

  • Aerobic capacity
  • Antioxidant
  • Carnosine
  • Running
  • Sex
  • Supplement

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Exercise-induced oxidative stress : The effects of β-alanine supplementation in women. / Smith, A. E.; Stout, J. R.; Kendall, K. L.; Fukuda, D. H.; Cramer, J. T.

In: Amino Acids, Vol. 43, No. 1, 01.07.2012, p. 77-90.

Research output: Contribution to journalArticle

Smith, A. E. ; Stout, J. R. ; Kendall, K. L. ; Fukuda, D. H. ; Cramer, J. T. / Exercise-induced oxidative stress : The effects of β-alanine supplementation in women. In: Amino Acids. 2012 ; Vol. 43, No. 1. pp. 77-90.
@article{e93e99566efc4cfc96bb239cf328a1a2,
title = "Exercise-induced oxidative stress: The effects of β-alanine supplementation in women",
abstract = "The purpose of this study was to evaluate the effects of β-alanine supplementation on markers of oxidative stress. Twenty-four women (age: 21.7 ± 2.1 years; VO2max: 2.6 ± 0.3 l min-1) were randomly assigned, in a double-blind fashion, to a β-alanine (BA, 2 × 800 mg tablets, 3× daily; CarnoSyn{\circledR}; n = 13) or placebo (PL, 2 × 800 mg maltodextrin tablets, 3× daily; n = 11) group. A graded oxygen consumption test (VO2max) was performed to evaluate VO2max, time to exhaustion, ventilatory threshold and establish peak velocity (PV). A 40-min treadmill run was used to induce oxidative stress. Total antioxidant capacity, superoxide dismutase, 8-isoprostane (8ISO) and reduced glutathione were measured. Heart rate and ratings of perceived exertion were recorded during the 40 min run. Separate three- [4 × 2 × 2; acute (base vs. IP vs. 2 vs. 4 h) × chronic (pre- vs. post-) × treatment (BA vs. PL)] and two- [2 × 2; time (pre-supplement vs. post-supplement) × treatment (BA vs. PL)] way ANOVAs were used for analyses. There was a significant increase in VO2max (p = 0.009), independent of treatment, with no significant changes in TTE (p = 0.074) or VT (p = 0.344). Ratings of perceived exertion values were significantly improved from pre- to post-supplementation for the BA group only at 40 min (p = 0.02). The ANOVA model demonstrated no significant treatment effects on oxidative stress. The chronic effects of BA supplementation demonstrated little antioxidant potential, in women, and little influence on aerobic performance assessments.",
keywords = "Aerobic capacity, Antioxidant, Carnosine, Running, Sex, Supplement",
author = "Smith, {A. E.} and Stout, {J. R.} and Kendall, {K. L.} and Fukuda, {D. H.} and Cramer, {J. T.}",
year = "2012",
month = "7",
day = "1",
doi = "10.1007/s00726-011-1158-x",
language = "English (US)",
volume = "43",
pages = "77--90",
journal = "Amino Acids",
issn = "0939-4451",
publisher = "Springer Wien",
number = "1",

}

TY - JOUR

T1 - Exercise-induced oxidative stress

T2 - The effects of β-alanine supplementation in women

AU - Smith, A. E.

AU - Stout, J. R.

AU - Kendall, K. L.

AU - Fukuda, D. H.

AU - Cramer, J. T.

PY - 2012/7/1

Y1 - 2012/7/1

N2 - The purpose of this study was to evaluate the effects of β-alanine supplementation on markers of oxidative stress. Twenty-four women (age: 21.7 ± 2.1 years; VO2max: 2.6 ± 0.3 l min-1) were randomly assigned, in a double-blind fashion, to a β-alanine (BA, 2 × 800 mg tablets, 3× daily; CarnoSyn®; n = 13) or placebo (PL, 2 × 800 mg maltodextrin tablets, 3× daily; n = 11) group. A graded oxygen consumption test (VO2max) was performed to evaluate VO2max, time to exhaustion, ventilatory threshold and establish peak velocity (PV). A 40-min treadmill run was used to induce oxidative stress. Total antioxidant capacity, superoxide dismutase, 8-isoprostane (8ISO) and reduced glutathione were measured. Heart rate and ratings of perceived exertion were recorded during the 40 min run. Separate three- [4 × 2 × 2; acute (base vs. IP vs. 2 vs. 4 h) × chronic (pre- vs. post-) × treatment (BA vs. PL)] and two- [2 × 2; time (pre-supplement vs. post-supplement) × treatment (BA vs. PL)] way ANOVAs were used for analyses. There was a significant increase in VO2max (p = 0.009), independent of treatment, with no significant changes in TTE (p = 0.074) or VT (p = 0.344). Ratings of perceived exertion values were significantly improved from pre- to post-supplementation for the BA group only at 40 min (p = 0.02). The ANOVA model demonstrated no significant treatment effects on oxidative stress. The chronic effects of BA supplementation demonstrated little antioxidant potential, in women, and little influence on aerobic performance assessments.

AB - The purpose of this study was to evaluate the effects of β-alanine supplementation on markers of oxidative stress. Twenty-four women (age: 21.7 ± 2.1 years; VO2max: 2.6 ± 0.3 l min-1) were randomly assigned, in a double-blind fashion, to a β-alanine (BA, 2 × 800 mg tablets, 3× daily; CarnoSyn®; n = 13) or placebo (PL, 2 × 800 mg maltodextrin tablets, 3× daily; n = 11) group. A graded oxygen consumption test (VO2max) was performed to evaluate VO2max, time to exhaustion, ventilatory threshold and establish peak velocity (PV). A 40-min treadmill run was used to induce oxidative stress. Total antioxidant capacity, superoxide dismutase, 8-isoprostane (8ISO) and reduced glutathione were measured. Heart rate and ratings of perceived exertion were recorded during the 40 min run. Separate three- [4 × 2 × 2; acute (base vs. IP vs. 2 vs. 4 h) × chronic (pre- vs. post-) × treatment (BA vs. PL)] and two- [2 × 2; time (pre-supplement vs. post-supplement) × treatment (BA vs. PL)] way ANOVAs were used for analyses. There was a significant increase in VO2max (p = 0.009), independent of treatment, with no significant changes in TTE (p = 0.074) or VT (p = 0.344). Ratings of perceived exertion values were significantly improved from pre- to post-supplementation for the BA group only at 40 min (p = 0.02). The ANOVA model demonstrated no significant treatment effects on oxidative stress. The chronic effects of BA supplementation demonstrated little antioxidant potential, in women, and little influence on aerobic performance assessments.

KW - Aerobic capacity

KW - Antioxidant

KW - Carnosine

KW - Running

KW - Sex

KW - Supplement

UR - http://www.scopus.com/inward/record.url?scp=84862763841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862763841&partnerID=8YFLogxK

U2 - 10.1007/s00726-011-1158-x

DO - 10.1007/s00726-011-1158-x

M3 - Article

C2 - 22102056

AN - SCOPUS:84862763841

VL - 43

SP - 77

EP - 90

JO - Amino Acids

JF - Amino Acids

SN - 0939-4451

IS - 1

ER -