Exercise ameliorates high fat diet induced cardiac dysfunction by increasing interleukin 10

Varun Kesherwani, Vishalakshi Chavali, Bryan T. Hackfort, Suresh C. Tyagi, Paras Kumar Mishra

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Increasing evidence suggests that a sedentary lifestyle and a high fat diet (HFD) leads to cardiomyopathy. Moderate exercise ameliorates cardiac dysfunction, however underlying molecular mechanisms are poorly understood. Increased inflammation due to induction of pro-inflammatory cytokine such as tumor necrosis factor-alpha (TNF-a) and attenuation of anti-inflammatory cytokine such as interleukin 10 (IL-10) contributes to cardiac dysfunction in obese and diabetics. We hypothesized that exercise training ameliorates HFD- induced cardiac dysfunction by mitigating obesity and inflammation through upregulation of IL-10 and downregulation of TNF-a. To test this hypothesis, 8 week old, female C57BL/6J mice were fed with HFD and exercised (swimming 1 h/day for 5 days/week for 8 weeks). The four treatment groups: normal diet (ND), HFD, HFD + exercise (HFD + Ex) and ND + Ex were analyzed for mean body weight, blood glucose level, TNF-a, IL-10, cardiac fibrosis by Masson Trichrome, and cardiac dysfunction by echocardiography. Mean body weights were increased in HFD but comparatively less in HFD + Ex. The level of TNF-a was elevated and IL-10 was downregulated in HFD but ameliorated in HFD + Ex. Cardiac fibrosis increased in HFD and was attenuated by exercise in the HFD + Ex group. The percentage ejection fraction and fractional shortening were decreased in HFD but comparatively increased in HFD + Ex. There was no difference between ND and ND + Ex for the above parameters except an increase in IL-10 level following exercise. Based on these results, we conclude that exercise mitigates HFD- induced cardiomyopathy by decreasing obesity, inducing IL-10, and reducing TNF-a in mice.

Original languageEnglish (US)
Article number124
JournalFrontiers in Physiology
Volume6
Issue numberAPR
DOIs
StatePublished - Jan 1 2015

Fingerprint

High Fat Diet
Interleukin-10
Tumor Necrosis Factor-alpha
Diet
Cardiomyopathies
Fibrosis
Down-Regulation
Obesity
Body Weight
Cytokines
Inflammation
Sedentary Lifestyle
Inbred C57BL Mouse
Echocardiography
Blood Glucose

Keywords

  • Echocardiography
  • Heart failure
  • Inflammation
  • Interleukin 10
  • Masson Trichrome
  • Obesity
  • TNF-a

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Exercise ameliorates high fat diet induced cardiac dysfunction by increasing interleukin 10. / Kesherwani, Varun; Chavali, Vishalakshi; Hackfort, Bryan T.; Tyagi, Suresh C.; Mishra, Paras Kumar.

In: Frontiers in Physiology, Vol. 6, No. APR, 124, 01.01.2015.

Research output: Contribution to journalArticle

Kesherwani, Varun ; Chavali, Vishalakshi ; Hackfort, Bryan T. ; Tyagi, Suresh C. ; Mishra, Paras Kumar. / Exercise ameliorates high fat diet induced cardiac dysfunction by increasing interleukin 10. In: Frontiers in Physiology. 2015 ; Vol. 6, No. APR.
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