Examination of Staphylococcus epidermidis biofilms using flow-cell technology

Derek E. Moormeier, Kenneth W Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

A common in vitro method to study Staphylococcus epidermidis biofilm development is to allow the bacteria to attach and grow on a solid surface in the presence of a continuous flow of nutrients. Under these conditions, the bacteria progress through a series of developmental steps, ultimately forming a multicellular structure containing differentiated cell populations. The observation of the biofilm at various time-points throughout this process provides a glimpse of the temporal changes that occur. Furthermore, use of metabolic stains and fluorescent reporters provides insight into the physiologic and transcriptional changes that occur within a developing biofilm. Currently, there are multiple systems available to assess biofilm development, each with advantages and disadvantages depending on the questions being asked. In this chapter, we describe the use of two separate flow-cell systems used to evaluate the developmental characteristics of staphylococcal biofilms: the FC270 flow-cell system from BioSurface Technologies, Corp. and the BioFlux1000 microfluidic flow-cell system from Fluxion Bioscience, Inc.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-155
Number of pages13
Volume1106
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)10643745

Fingerprint

Staphylococcus epidermidis
Biofilms
Technology
Bacteria
Microfluidics
Coloring Agents
Observation
Food
Population

Keywords

  • Biofilm
  • BioFlux
  • Confocal laser scanning microscopy
  • Epifluorescence microscopy
  • Flow-cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Moormeier, D. E., & Bayles, K. W. (2014). Examination of Staphylococcus epidermidis biofilms using flow-cell technology. In Methods in Molecular Biology (Vol. 1106, pp. 143-155). (Methods in Molecular Biology; Vol. 1106). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-736-5_13

Examination of Staphylococcus epidermidis biofilms using flow-cell technology. / Moormeier, Derek E.; Bayles, Kenneth W.

Methods in Molecular Biology. Vol. 1106 Humana Press Inc., 2014. p. 143-155 (Methods in Molecular Biology; Vol. 1106).

Research output: Chapter in Book/Report/Conference proceedingChapter

Moormeier, DE & Bayles, KW 2014, Examination of Staphylococcus epidermidis biofilms using flow-cell technology. in Methods in Molecular Biology. vol. 1106, Methods in Molecular Biology, vol. 1106, Humana Press Inc., pp. 143-155. https://doi.org/10.1007/978-1-62703-736-5_13
Moormeier DE, Bayles KW. Examination of Staphylococcus epidermidis biofilms using flow-cell technology. In Methods in Molecular Biology. Vol. 1106. Humana Press Inc. 2014. p. 143-155. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-736-5_13
Moormeier, Derek E. ; Bayles, Kenneth W. / Examination of Staphylococcus epidermidis biofilms using flow-cell technology. Methods in Molecular Biology. Vol. 1106 Humana Press Inc., 2014. pp. 143-155 (Methods in Molecular Biology).
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