Evolutionary relationship of three mitten crabs (Eriocheir sp)revealed by mitogenome and 5S ribosomal DNA analysis

Xiaowen Chen, Jun Wang, Lei Huang, Wucheng Yue, Jixing Zou, Chunhong Yuan, Guoqing Lu, Chenghui Wang

Research output: Contribution to journalArticle

Abstract

To reveal the evolutionary relationship of three mitten crabs (Eriocheir sinensis, E. hepuensis, and E. japonica), complete mitogenomes and nuclear 5S rDNA sequences were analyzed. Sequencing revealed that the mitogenomes analyzed shared conserved organization of the coding and non-coding regions but genetic variation was identified. Among the three mitten crabs distinct tandem repeats were identified in the mitochondrial D-loop region. The 5S gene (5S rDNA)sequence was highly conserved across the three species, whereas non-transcribed spacer (NTS)region exhibit high levels of variation including insertions, deletions and point mutations. Cluster analysis suggested that the three mitten crabs had their own independent 5S rDNA sequence variation and evolutionary pattern. Both mitogenome and 5S rDNA sequence analysis revealed significant genetic variation across the mitten crabs species. Phylogenetic analysis using mitogenome and 5S rDNA sequences demonstrated that E. japonica was relatively more distant from E. sinensis and E. hepuensis. This study extended our previous knowledge and confirmed that the three mitten crabs are likely to be genetically differentiated species. In addition, our study also provided insights into the conservation of pure natural resources of E. sinensis, an important aquaculture species.

Original languageEnglish (US)
Pages (from-to)256-261
Number of pages6
JournalAquaculture and Fisheries
Volume2
Issue number6
DOIs
StatePublished - Nov 2017

Fingerprint

Eriocheir
ribosomal DNA
crab
crabs
Eriocheir sinensis
DNA
genetic variation
tandem repeat sequences
point mutation
natural resources
aquaculture
cluster analysis
mutation
natural resource
sequence analysis
mitochondrial genome
analysis
phylogenetics
gene
phylogeny

Keywords

  • 5S rDNA
  • Evolutionary relationship
  • Mitochondrial genome
  • Mitten crabs

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Evolutionary relationship of three mitten crabs (Eriocheir sp)revealed by mitogenome and 5S ribosomal DNA analysis. / Chen, Xiaowen; Wang, Jun; Huang, Lei; Yue, Wucheng; Zou, Jixing; Yuan, Chunhong; Lu, Guoqing; Wang, Chenghui.

In: Aquaculture and Fisheries, Vol. 2, No. 6, 11.2017, p. 256-261.

Research output: Contribution to journalArticle

Chen, Xiaowen ; Wang, Jun ; Huang, Lei ; Yue, Wucheng ; Zou, Jixing ; Yuan, Chunhong ; Lu, Guoqing ; Wang, Chenghui. / Evolutionary relationship of three mitten crabs (Eriocheir sp)revealed by mitogenome and 5S ribosomal DNA analysis. In: Aquaculture and Fisheries. 2017 ; Vol. 2, No. 6. pp. 256-261.
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