Evolutionary and functional insights into the mechanism underlying high-altitude adaptation of deer mouse hemoglobin

Jay F Storz, Amy M. Runck, Stephen J. Sabatino, John K. Kelly, Nuno Ferrand, Hideaki Moriyama, Roy E. Weber, Angela Fago

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Adaptive modifications of heteromeric proteins may involve genetically based changes in single subunit polypeptides or parallel changes in multiple genes that encode distinct, interacting subunits. Here we investigate these possibilities by conducting a combined evolutionary and functional analysis of duplicated globin genes in natural populations of deer mice (Peromyscus maniculatus) that are adapted to different elevational zones. A multilocus analysis of nucleotide polymorphism and linkage disequilibrium revealed that high-altitude adaptation of deer mouse hemoglobin involves parallel functional differentiation at multiple unlinked gene duplicates: two α-globin paralogs on chromosome 8 and two β-globin paralogs on chromosome 1. Differences in O2-binding affinity of the alternative β-chain hemoglobin isoforms were entirely attributable to allelic differences in sensitivity to 2,3-diphosphoglycerate (DPG), an allosteric cofactor that stabilizes the low-affinity, deoxygenated conformation of the hemoglobin tetramer. The two-locus β-globin haplotype that predominates at high altitude is associated with suppressed DPGsensitivity (and hence, increased hemoglobin-O2 affinity), which enhances pulmonary O2 loading under hypoxia. The discovery that allelic differences in DPG-sensitivity contribute to adaptive variation in hemoglobin-O2 affinity illustrates the value of integrating evolutionary analyses of sequence variation with mechanistic appraisals of protein function. Investigation into the functional significance of the deer mouse β-globin polymorphism was motivated by the results of population genetic analyses which revealed evidence for a history of divergent selection between elevational zones. The experimental measures of O2-binding properties corroborated the tests of selection by demonstrating a functional difference between the products of alternative alleles.

Original languageEnglish (US)
Pages (from-to)14450-14455
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number34
DOIs
StatePublished - Aug 25 2009

Fingerprint

Peromyscus
Globins
Hemoglobins
2,3-Diphosphoglycerate
Duplicate Genes
Chromosomes, Human, Pair 8
Chromosomes, Human, Pair 1
Linkage Disequilibrium
Population Genetics
Haplotypes
Genes
Sequence Analysis
Protein Isoforms
Proteins
Nucleotides
Alleles
Lung
Peptides
Population

Keywords

  • Gene duplication
  • Hypoxia
  • Molecular adaptation
  • Peromyscus
  • Positive selection

ASJC Scopus subject areas

  • General

Cite this

Evolutionary and functional insights into the mechanism underlying high-altitude adaptation of deer mouse hemoglobin. / Storz, Jay F; Runck, Amy M.; Sabatino, Stephen J.; Kelly, John K.; Ferrand, Nuno; Moriyama, Hideaki; Weber, Roy E.; Fago, Angela.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 34, 25.08.2009, p. 14450-14455.

Research output: Contribution to journalArticle

Storz, Jay F ; Runck, Amy M. ; Sabatino, Stephen J. ; Kelly, John K. ; Ferrand, Nuno ; Moriyama, Hideaki ; Weber, Roy E. ; Fago, Angela. / Evolutionary and functional insights into the mechanism underlying high-altitude adaptation of deer mouse hemoglobin. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 34. pp. 14450-14455.
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