Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula

Chenbing Ai, Samuel McCarthy, Yuting Liang, Deepak Rudrappa, Guanzhou Qiu, Paul Blum

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula ARS50-2, contained 12 and 13 additional mutations, respectively, relative to M. sedula CuR1. Bioleaching capacity of a defined consortium (consisting of a naturally occurring strain and a genetically engineered copper sensitive strain) was increased by introduction of M. sedula ARS50-2, with 5.31 and 26.29% more copper recovered from enargite at a pulp density (PD) of 1 and 3% (w/v), respectively. M. sedula ARS50-2 arose as the predominant species and modulated the proportions of the other two strains after it had been introduced. Collectively, the higher Cu2+ resistance trait of M. sedula ARS50-2 resulted in a modulated microbial community structure, and consolidating enargite bioleaching especially at elevated PD.

Original languageEnglish (US)
Pages (from-to)1613-1625
Number of pages13
JournalJournal of Industrial Microbiology and Biotechnology
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

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Keywords

  • Arsenate resistance
  • Enargite bioleaching
  • Extreme thermoacidophile
  • Metallosphaera sedula
  • Mutation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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