Evolution of amber suppressor tRNAs for efficient bacterial production of proteins containing nonnatural amino acids

Jiantao Guo, Charles E. Melançon, Hyun Soo Lee, Dan Groff, Peter G. Schultz

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Applied evolution: Regions of the M.jannaschii tyrosyl tRNACUA thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved yields of proteins containing nonnatural amino acids. In some cases, the degree of improvement varied in an amino acid dependent manner.

Original languageEnglish (US)
Pages (from-to)9148-9151
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number48
DOIs
StatePublished - Nov 16 2009

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Keywords

  • Amber suppression
  • Evolution
  • Nonnatural amino acids
  • Protein modifications
  • TRNA

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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