Evolution of amber suppressor tRNAs for efficient bacterial production of proteins containing nonnatural amino acids

Jiantao Guo, Charles E. Melançon, Hyun Soo Lee, Dan Groff, Peter G. Schultz

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Applied evolution: Regions of the M.jannaschii tyrosyl tRNACUA thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved yields of proteins containing nonnatural amino acids. In some cases, the degree of improvement varied in an amino acid dependent manner.

Original languageEnglish (US)
Pages (from-to)9148-9151
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number48
DOIs
StatePublished - Nov 16 2009

Fingerprint

Transfer RNA
Amino acids
Peptide Elongation Factor Tu
Proteins
Amino Acids
Elongation

Keywords

  • Amber suppression
  • Evolution
  • Nonnatural amino acids
  • Protein modifications
  • TRNA

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Evolution of amber suppressor tRNAs for efficient bacterial production of proteins containing nonnatural amino acids. / Guo, Jiantao; Melançon, Charles E.; Lee, Hyun Soo; Groff, Dan; Schultz, Peter G.

In: Angewandte Chemie - International Edition, Vol. 48, No. 48, 16.11.2009, p. 9148-9151.

Research output: Contribution to journalArticle

Guo, Jiantao ; Melançon, Charles E. ; Lee, Hyun Soo ; Groff, Dan ; Schultz, Peter G. / Evolution of amber suppressor tRNAs for efficient bacterial production of proteins containing nonnatural amino acids. In: Angewandte Chemie - International Edition. 2009 ; Vol. 48, No. 48. pp. 9148-9151.
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