Evolution and diversification of lamprey antigen receptors: Evidence for involvement of an AID-APOBEC family cytosine deaminase

Igor B. Rogozin, Lakshminarayan M. Iyer, Lizhi Liang, Galina V. Glazko, Victoria G. Liston, Youri I Pavlov, L. Aravind, Zeev Pancer

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

The variable lymphocyte receptors (VLRs) of jawless vertebrates such as lamprey and hagfish are composed of highly diverse modular leucine-rich repeats. Each lymphocyte assembles a unique VLR by rearrangement of the germline gene. In the lamprey genome, we identify here about 850 distinct cassettes encoding leucine-rich repeat modules that serve as sequence templates for the hypervariable VLR repertoires. The data indicate a gene conversion-like process in VLR diversification. Genomic analysis suggested a link between the VLR and platelet glycoprotein receptors. Lamprey lymphocytes express two putative deaminases of the AID-APOBEC family that may be involved in VLR diversification, as indicated by in vitro mutagenesis and recombination assays. Vertebrate acquired immunity could have therefore originated from lymphocyte receptor diversification by an ancestral AID-like DNA cytosine deaminase.

Original languageEnglish (US)
Pages (from-to)647-656
Number of pages10
JournalNature Immunology
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2007

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Cytosine Deaminase
Lampreys
Antigen Receptors
Lymphocytes
Leucine
Vertebrates
Hagfishes
Platelet Membrane Glycoproteins
Gene Conversion
Gene Rearrangement
Adaptive Immunity
Mutagenesis
Genetic Recombination
Genome

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Evolution and diversification of lamprey antigen receptors : Evidence for involvement of an AID-APOBEC family cytosine deaminase. / Rogozin, Igor B.; Iyer, Lakshminarayan M.; Liang, Lizhi; Glazko, Galina V.; Liston, Victoria G.; Pavlov, Youri I; Aravind, L.; Pancer, Zeev.

In: Nature Immunology, Vol. 8, No. 6, 01.06.2007, p. 647-656.

Research output: Contribution to journalArticle

Rogozin, Igor B. ; Iyer, Lakshminarayan M. ; Liang, Lizhi ; Glazko, Galina V. ; Liston, Victoria G. ; Pavlov, Youri I ; Aravind, L. ; Pancer, Zeev. / Evolution and diversification of lamprey antigen receptors : Evidence for involvement of an AID-APOBEC family cytosine deaminase. In: Nature Immunology. 2007 ; Vol. 8, No. 6. pp. 647-656.
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