Evidence That Intravenously Derived Murine Pulmonary Melanoma Metastases Can Originate from the Expansion of a Single Tumor Cell

Isaiah J. Fidler, James E. Talmadge

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

The purpose of these studies was to determine whether hematogenous clonal pulmonary melanoma metastases originate from the expansion of a single cell and if so, by extrapolation, metastasis can be considered a cloning process. Three different cell lines of murine K-1735 melanoma with different metastatic properties and unique karyotypes were injected i.v. into syngeneic C3H/HeN mice as multicell aggregates of individual cell lines or combinations of cell lines. Resultant solitary lung metastases were isolated in culture as individual lines and then karyotyped. Even when heterogeneous clumps of tumor cells were injected, the individual metastases exhibited a karyotype unique to one metastatic cell type. Furthermore, when cellular aggregates were composed of metastatic cells admixed with cells that were tumorigenic but nonmetastatic, the resultant metastases exhibited only the karyotype of the metastatic cells. This finding suggests that the presence of metastatic cells did not change the inability of nonmetastatic cells to proliferate in a distant organ. Collectively, the results indicate that the resultant metastases were of clonal origin owing to the expansion of a single metastatic tumor cell in the lung parenchyma.

Original languageEnglish (US)
Pages (from-to)5167-5171
Number of pages5
JournalCancer Research
Volume46
Issue number10
StatePublished - Oct 1 1986

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Melanoma
Neoplasm Metastasis
Lung
Neoplasms
Karyotype
Cell Line
Inbred C3H Mouse
Organism Cloning

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Evidence That Intravenously Derived Murine Pulmonary Melanoma Metastases Can Originate from the Expansion of a Single Tumor Cell. / Fidler, Isaiah J.; Talmadge, James E.

In: Cancer Research, Vol. 46, No. 10, 01.10.1986, p. 5167-5171.

Research output: Contribution to journalArticle

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abstract = "The purpose of these studies was to determine whether hematogenous clonal pulmonary melanoma metastases originate from the expansion of a single cell and if so, by extrapolation, metastasis can be considered a cloning process. Three different cell lines of murine K-1735 melanoma with different metastatic properties and unique karyotypes were injected i.v. into syngeneic C3H/HeN mice as multicell aggregates of individual cell lines or combinations of cell lines. Resultant solitary lung metastases were isolated in culture as individual lines and then karyotyped. Even when heterogeneous clumps of tumor cells were injected, the individual metastases exhibited a karyotype unique to one metastatic cell type. Furthermore, when cellular aggregates were composed of metastatic cells admixed with cells that were tumorigenic but nonmetastatic, the resultant metastases exhibited only the karyotype of the metastatic cells. This finding suggests that the presence of metastatic cells did not change the inability of nonmetastatic cells to proliferate in a distant organ. Collectively, the results indicate that the resultant metastases were of clonal origin owing to the expansion of a single metastatic tumor cell in the lung parenchyma.",
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