Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8- oxoguanine) in human cells

T. Bessho, K. Tano, H. Kasai, E. Ohtsuka, S. Nishimura

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Two DNA repair enzymes for 8-hydroxyguanine (also known as 7,8-dihydro-8- oxoguanine; OH8Gua, oxo8Gua) have been identified in human HeLa cell nuclear extract. One is OH8Gua-glycosylase and the other is OH8Gua- endonuclease that lacks OH8Gua-glycosylase activity. They were separated by heparin-Sepharose column chromatography and characterized by endonuclease nicking assay or by measuring the OH8Gua released from substrate DNA using high pressure liquid chromatography-electrochemical detection. Both OH8Gua repair enzymes act only on the OH8Gua-containing strand of the duplex substrate DNA containing OH8Gua/C, OH8Gua/T, or OH8Gua/G. DNA containing OH8Gua/A base pair was very poor substrate for either enzymes. OH8Gua- endonuclease simultaneously cleaves phosphodiester bonds on both sides of the OH8Gua residue, leaving 5'-hydroxy and 3'-hydroxy groups.

Original languageEnglish (US)
Pages (from-to)19416-19421
Number of pages6
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - Jan 1 1993

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DNA Repair Enzymes
Endonucleases
Cells
DNA
Substrates
High pressure liquid chromatography
Agarose Chromatography
Column chromatography
Enzymes
Cell Extracts
HeLa Cells
Base Pairing
Assays
Repair
High Pressure Liquid Chromatography
7,8-dihydro-8-oxoguanine
8-hydroxyguanine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8- oxoguanine) in human cells. / Bessho, T.; Tano, K.; Kasai, H.; Ohtsuka, E.; Nishimura, S.

In: Journal of Biological Chemistry, Vol. 268, No. 26, 01.01.1993, p. 19416-19421.

Research output: Contribution to journalArticle

Bessho, T, Tano, K, Kasai, H, Ohtsuka, E & Nishimura, S 1993, 'Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8- oxoguanine) in human cells', Journal of Biological Chemistry, vol. 268, no. 26, pp. 19416-19421.
Bessho, T. ; Tano, K. ; Kasai, H. ; Ohtsuka, E. ; Nishimura, S. / Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8- oxoguanine) in human cells. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 26. pp. 19416-19421.
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