Evidence for the lack of serotonergic mechanism in the regulation of blood-brain barrier

Anil Gulati, Sandeep Kumar Agrawal, Rakesh Shukla, Rikhab C. Srimal, Bhola N. Dhawan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Role of serotonergic mechanism in the regulation of blood-brain barrier in anaesthetised cats and dogs has been investigated. In cats, the circulant used was Evans blue. The drugs as well as the circulant were administered through the left carotid artery. The staining of the brain substance was scored at autopsy. 5-Hydroxytryptamine (5-HT), 5-hydroxytryptophan (5-HTP), 5-HT uptake-inhibitors, citalopram and fluvoxamine, as well as normal saline produced no staining whereas hypertonic sodium chloride solution produced significant staining of the brain substance. In experiments on dogs, sodium fluorescein was injected intravenously and the cerebrospinal fluid (CSF) was collected through a needle put in the cisterna magna for estimation of the dye. Normal saline, 5-HT, citalopram, fluvoxamine and methysergide failed to affect the entry of sodium fluorescein in the CSF while hypertonic sodium chloride solution increased it significantly. Furthermore, 5-HT did not affect the increased entry of sodium fluorescein induced by hypertonic sodium chloride. It is concluded that serotonergic mechanism does not play a significant role in the regulation of blood-brain barrier.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalPharmacological Research Communications
Volume16
Issue number2
DOIs
StatePublished - Jan 1 1984

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Blood-Brain Barrier
Fluorescein
Hypertonic Saline Solutions
Fluvoxamine
Serotonin
Citalopram
Staining and Labeling
Cerebrospinal Fluid
Cats
Dogs
Cisterna Magna
Methysergide
5-Hydroxytryptophan
Evans Blue
Serotonin Uptake Inhibitors
Brain
Carotid Arteries
Sodium Chloride
Needles
Autopsy

ASJC Scopus subject areas

  • Pharmacology

Cite this

Evidence for the lack of serotonergic mechanism in the regulation of blood-brain barrier. / Gulati, Anil; Agrawal, Sandeep Kumar; Shukla, Rakesh; Srimal, Rikhab C.; Dhawan, Bhola N.

In: Pharmacological Research Communications, Vol. 16, No. 2, 01.01.1984, p. 181-188.

Research output: Contribution to journalArticle

Gulati, Anil ; Agrawal, Sandeep Kumar ; Shukla, Rakesh ; Srimal, Rikhab C. ; Dhawan, Bhola N. / Evidence for the lack of serotonergic mechanism in the regulation of blood-brain barrier. In: Pharmacological Research Communications. 1984 ; Vol. 16, No. 2. pp. 181-188.
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