Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links

Laura Zietlow, Leigh Anne Smith, Mika Bessho, Tadayoshi Bessho

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Human DNA polymerase N (PolN) is an A-family nuclear DNA polymerase whose function is unknown. This study examines the possible role of PolN in DNA repair in human cells treated with PolN-targeted siRNA. HeLa cells with siRNA-mediated knockdown of PolN were more sensitive than control cells to DNA cross-linking agent mitomycin C (MMC) but were not hypersensitive to UV irradiation. The MMC hypersensitivity of PolN knockdown cells was rescued by the overexpression of DNA polymerase-proficient PolN but not by DNA polymerase-deficient PolN. Furthermore, in vitro experiments showed that purified PolN conducts low-efficiency nonmutagenic bypass of a psoralen DNA interstrand cross-link (ICL), whose structure resembles an intermediate in the proposed pathway of ICL repair. These results suggest that PolN might play a role in translesion DNA synthesis during ICL repair in human cells.

Original languageEnglish (US)
Pages (from-to)11817-11824
Number of pages8
JournalBiochemistry
Volume48
Issue number49
DOIs
StatePublished - Dec 15 2009

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DNA Repair
Repair
DNA-Directed DNA Polymerase
DNA
Mitomycin
Small Interfering RNA
Cells
Ficusin
Nuclear Family
HeLa Cells
Hypersensitivity
Irradiation
human DNA polymerase N
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links. / Zietlow, Laura; Smith, Leigh Anne; Bessho, Mika; Bessho, Tadayoshi.

In: Biochemistry, Vol. 48, No. 49, 15.12.2009, p. 11817-11824.

Research output: Contribution to journalArticle

Zietlow, Laura ; Smith, Leigh Anne ; Bessho, Mika ; Bessho, Tadayoshi. / Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links. In: Biochemistry. 2009 ; Vol. 48, No. 49. pp. 11817-11824.
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