Evidence for the clonal origin of spontaneous metastases

James E. Talmadge, Sandra R. Wolman, Isaiah J. Fidler

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

A cultured cell line of the K-1735 melanoma was x-irradiated to induce chromosome breakage and rearrangements and then was implanted into the footpads of syngenic C3H mice. Spontaneous lung metastases were isolated from different animals, established in culture as individual lines, and then karyotyped. Within certain metastases, the same chromosomal abnormality (or abnormalities) (recombinant chromosomes) was found in all the cells examined. Most metastases differed from one another in that they exhibited characteristic combinations of chromosomal markers. These findings indicated that the metastases were clonal and that they probably originated from different progenitor cells.

Original languageEnglish (US)
Pages (from-to)361-363
Number of pages3
JournalScience
Volume217
Issue number4557
DOIs
StatePublished - Jan 1 1982

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Neoplasm Metastasis
Chromosome Aberrations
Chromosome Breakage
Inbred C3H Mouse
Cultured Cells
Melanoma
Stem Cells
Cell Line
Lung

ASJC Scopus subject areas

  • General

Cite this

Evidence for the clonal origin of spontaneous metastases. / Talmadge, James E.; Wolman, Sandra R.; Fidler, Isaiah J.

In: Science, Vol. 217, No. 4557, 01.01.1982, p. 361-363.

Research output: Contribution to journalArticle

Talmadge, James E. ; Wolman, Sandra R. ; Fidler, Isaiah J. / Evidence for the clonal origin of spontaneous metastases. In: Science. 1982 ; Vol. 217, No. 4557. pp. 361-363.
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