Abstract
A cultured cell line of the K-1735 melanoma was x-irradiated to induce chromosome breakage and rearrangements and then was implanted into the footpads of syngenic C3H mice. Spontaneous lung metastases were isolated from different animals, established in culture as individual lines, and then karyotyped. Within certain metastases, the same chromosomal abnormality (or abnormalities) (recombinant chromosomes) was found in all the cells examined. Most metastases differed from one another in that they exhibited characteristic combinations of chromosomal markers. These findings indicated that the metastases were clonal and that they probably originated from different progenitor cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 361-363 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 217 |
| Issue number | 4557 |
| DOIs | |
| State | Published - Jan 1 1982 |
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Evidence for the clonal origin of spontaneous metastases. / Talmadge, James E.; Wolman, Sandra R.; Fidler, Isaiah J.
In: Science, Vol. 217, No. 4557, 01.01.1982, p. 361-363.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for the clonal origin of spontaneous metastases
AU - Talmadge, James E.
AU - Wolman, Sandra R.
AU - Fidler, Isaiah J.
PY - 1982/1/1
Y1 - 1982/1/1
N2 - A cultured cell line of the K-1735 melanoma was x-irradiated to induce chromosome breakage and rearrangements and then was implanted into the footpads of syngenic C3H mice. Spontaneous lung metastases were isolated from different animals, established in culture as individual lines, and then karyotyped. Within certain metastases, the same chromosomal abnormality (or abnormalities) (recombinant chromosomes) was found in all the cells examined. Most metastases differed from one another in that they exhibited characteristic combinations of chromosomal markers. These findings indicated that the metastases were clonal and that they probably originated from different progenitor cells.
AB - A cultured cell line of the K-1735 melanoma was x-irradiated to induce chromosome breakage and rearrangements and then was implanted into the footpads of syngenic C3H mice. Spontaneous lung metastases were isolated from different animals, established in culture as individual lines, and then karyotyped. Within certain metastases, the same chromosomal abnormality (or abnormalities) (recombinant chromosomes) was found in all the cells examined. Most metastases differed from one another in that they exhibited characteristic combinations of chromosomal markers. These findings indicated that the metastases were clonal and that they probably originated from different progenitor cells.
UR - http://www.scopus.com/inward/record.url?scp=0019983407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019983407&partnerID=8YFLogxK
U2 - 10.1126/science.6953592
DO - 10.1126/science.6953592
M3 - Article
C2 - 6953592
AN - SCOPUS:0019983407
VL - 217
SP - 361
EP - 363
JO - Science
JF - Science
SN - 0036-8075
IS - 4557
ER -