Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor

Woo Yang Kim, Zahra Fayazi, Xiankun Bao, Dennis Higgins, Parsa Kazemi-Esfarjani

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.

Original languageEnglish (US)
Pages (from-to)536-544
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume29
Issue number4
DOIs
StatePublished - Aug 1 2005

Fingerprint

Myeloid Leukemia
Drosophila
Huntington Disease
Drosophila melanogaster
Neurodegenerative Diseases
Phenotype
polyglutamine
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor. / Kim, Woo Yang; Fayazi, Zahra; Bao, Xiankun; Higgins, Dennis; Kazemi-Esfarjani, Parsa.

In: Molecular and Cellular Neuroscience, Vol. 29, No. 4, 01.08.2005, p. 536-544.

Research output: Contribution to journalArticle

Kim, Woo Yang ; Fayazi, Zahra ; Bao, Xiankun ; Higgins, Dennis ; Kazemi-Esfarjani, Parsa. / Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor. In: Molecular and Cellular Neuroscience. 2005 ; Vol. 29, No. 4. pp. 536-544.
@article{33f9a02f7579404799adb3f9a2ead48b,
title = "Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor",
abstract = "Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.",
author = "Kim, {Woo Yang} and Zahra Fayazi and Xiankun Bao and Dennis Higgins and Parsa Kazemi-Esfarjani",
year = "2005",
month = "8",
day = "1",
doi = "10.1016/j.mcn.2005.04.005",
language = "English (US)",
volume = "29",
pages = "536--544",
journal = "Molecular and Cellular Neurosciences",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor

AU - Kim, Woo Yang

AU - Fayazi, Zahra

AU - Bao, Xiankun

AU - Higgins, Dennis

AU - Kazemi-Esfarjani, Parsa

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.

AB - Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.

UR - http://www.scopus.com/inward/record.url?scp=22144471491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22144471491&partnerID=8YFLogxK

U2 - 10.1016/j.mcn.2005.04.005

DO - 10.1016/j.mcn.2005.04.005

M3 - Article

C2 - 15936212

AN - SCOPUS:22144471491

VL - 29

SP - 536

EP - 544

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

SN - 1044-7431

IS - 4

ER -