Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor

Woo Yang Kim, Zahra Fayazi, Xiankun Bao, Dennis Higgins, Parsa Kazemi-Esfarjani

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26 Scopus citations

Abstract

Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.

Original languageEnglish (US)
Pages (from-to)536-544
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume29
Issue number4
DOIs
StatePublished - Aug 1 2005

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ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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