Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2)

Patrick Masson, Arnaud Chatonnet, Oksana Lockridge

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C5 variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C5 is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalFEBS Letters
Volume262
Issue number1
DOIs
StatePublished - Mar 12 1990

Fingerprint

Butyrylcholinesterase
Cholinesterases
Genes
Plasmas
Plasma (human)
DNA
Southern Blotting
Exons
Leukocytes
Blood
Cells
Enzymes
Proteins

Keywords

  • (Human plasma)
  • Butyrylcholinesterase
  • CHE2
  • Southern blot analysis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2). / Masson, Patrick; Chatonnet, Arnaud; Lockridge, Oksana.

In: FEBS Letters, Vol. 262, No. 1, 12.03.1990, p. 115-118.

Research output: Contribution to journalArticle

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AB - DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C5 variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C5 is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.

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