Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.

P. J. Blackall, T. Yamaguchi, Y. Iritani, Douglas G Rogers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).

Original languageEnglish (US)
Pages (from-to)861-864
Number of pages4
JournalAvian diseases
Volume34
Issue number4
DOIs
StatePublished - Jan 1 1990

Fingerprint

Haemophilus paragallinarum
Avibacterium paragallinarum
Serotyping
monoclonal antibodies
serotypes
Monoclonal Antibodies
Serogroup
Hemagglutination Inhibition Tests
hemagglutination inhibition test
Agglutinins

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Blackall, P. J., Yamaguchi, T., Iritani, Y., & Rogers, D. G. (1990). Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. Avian diseases, 34(4), 861-864. https://doi.org/10.2307/1591374

Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. / Blackall, P. J.; Yamaguchi, T.; Iritani, Y.; Rogers, Douglas G.

In: Avian diseases, Vol. 34, No. 4, 01.01.1990, p. 861-864.

Research output: Contribution to journalArticle

Blackall, PJ, Yamaguchi, T, Iritani, Y & Rogers, DG 1990, 'Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.', Avian diseases, vol. 34, no. 4, pp. 861-864. https://doi.org/10.2307/1591374
Blackall, P. J. ; Yamaguchi, T. ; Iritani, Y. ; Rogers, Douglas G. / Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. In: Avian diseases. 1990 ; Vol. 34, No. 4. pp. 861-864.
@article{fbc191650fcb4f2ebd7182c6a5e993df,
title = "Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.",
abstract = "Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).",
author = "Blackall, {P. J.} and T. Yamaguchi and Y. Iritani and Rogers, {Douglas G}",
year = "1990",
month = "1",
day = "1",
doi = "10.2307/1591374",
language = "English (US)",
volume = "34",
pages = "861--864",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "4",

}

TY - JOUR

T1 - Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.

AU - Blackall, P. J.

AU - Yamaguchi, T.

AU - Iritani, Y.

AU - Rogers, Douglas G

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).

AB - Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).

UR - http://www.scopus.com/inward/record.url?scp=0025504245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025504245&partnerID=8YFLogxK

U2 - 10.2307/1591374

DO - 10.2307/1591374

M3 - Article

VL - 34

SP - 861

EP - 864

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 4

ER -