Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer

B. L. Williams, A. E.T. Sparks, R. S. Canseco, J. W. Knight, J. L. Johnson, W. H. Velander, R. L. Page, W. N. Drohan, E. T. Kornegay, R. E. Pearson, T. D. Wilkins, F. C. Gwazdauskas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 ± 2.9, 24.3 ± 1.8 vs 11.6 ± 2.7 ovulations; X ± SEM). The SALT and PRE treatments yielded 12.3 ± 2.6 and 17.7 ± 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation.

Original languageEnglish (US)
Pages (from-to)501-511
Number of pages11
JournalTheriogenology
Volume38
Issue number3
DOIs
StatePublished - Sep 1992

Fingerprint

Superovulation
superovulation
Zygote
Microinjections
zygote
human chorionic gonadotropin
Chorionic Gonadotropin
Equine Gonadotropins
Swine
gilts
pregnant mare serum gonadotropin
Ovulation
swine
Estrus Synchronization
DNA
sows
ovulation
estrus synchronization
Gene Transfer Techniques
Estrous Cycle

Keywords

  • estrus synchronization
  • pig
  • superovulation
  • transgenics

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Williams, B. L., Sparks, A. E. T., Canseco, R. S., Knight, J. W., Johnson, J. L., Velander, W. H., ... Gwazdauskas, F. C. (1992). Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer. Theriogenology, 38(3), 501-511. https://doi.org/10.1016/0093-691X(92)90070-8

Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer. / Williams, B. L.; Sparks, A. E.T.; Canseco, R. S.; Knight, J. W.; Johnson, J. L.; Velander, W. H.; Page, R. L.; Drohan, W. N.; Kornegay, E. T.; Pearson, R. E.; Wilkins, T. D.; Gwazdauskas, F. C.

In: Theriogenology, Vol. 38, No. 3, 09.1992, p. 501-511.

Research output: Contribution to journalArticle

Williams, BL, Sparks, AET, Canseco, RS, Knight, JW, Johnson, JL, Velander, WH, Page, RL, Drohan, WN, Kornegay, ET, Pearson, RE, Wilkins, TD & Gwazdauskas, FC 1992, 'Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer', Theriogenology, vol. 38, no. 3, pp. 501-511. https://doi.org/10.1016/0093-691X(92)90070-8
Williams, B. L. ; Sparks, A. E.T. ; Canseco, R. S. ; Knight, J. W. ; Johnson, J. L. ; Velander, W. H. ; Page, R. L. ; Drohan, W. N. ; Kornegay, E. T. ; Pearson, R. E. ; Wilkins, T. D. ; Gwazdauskas, F. C. / Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer. In: Theriogenology. 1992 ; Vol. 38, No. 3. pp. 501-511.
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