Evaluation of Rint1 as a modifier of intestinal tumorigenesis and cancer risk

Karla L. Otterpohl, Karen A Gould

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Rad50 Interacting Protein 1 (Rint1) influences cellular homeostasis through maintenance of endoplasmic reticulum, Golgi and centrosome integrity and regulation of vesicle transport, autophagy and the G2/M checkpoint. Rint1 has been postulated to function as a tumor suppressor as well as an oncogene, with its role depending perhaps upon the precise cellular and/or experimental context. In humans, heterozygosity for germline missense variants in RINT1 have, in some studies, been associated with increased risk of both breast and Lynch syndrome type cancers. However, it is not known if these germline variants represent loss of function alleles or gain of function alleles. Based upon these findings, as well as our initial consideration of Rint1 as a potential candidate for Mom5, a genetic modifier of intestinal tumorigenesis in ApcMin/+ mice, we sought to explicitly examine the impact of Rint1 on tumorigenesis in ApcMin/+ mice. However, heterozygosity for a knockout of Rint1 had no impact on tumorigenesis in Rint1+/-; ApcMin/+ mice. Likewise, we found no evidence to suggest that the remaining Rint1 allele was lost somatically in intestinal tumors in ApcMin/+ mice. Interestingly, in contrast to what has been observed in Rint1+/- mice on a mixed genetic background, Rint1+/- mice on a pure C57BL/6J background did not show spontaneous tumor development. We also evaluated colorectal cancer data available in the COSMIC and ONCOMINE databases and found that RINT1 overexpression, as well as the presence of somatic missense mutations in RINT1 were associated with colorectal cancer development. In vitro evaluation of two missense variants in RINT1 suggested that such variants do have the potential to impact RINT1 function.

Original languageEnglish (US)
Article numbere0172247
JournalPloS one
Volume12
Issue number3
DOIs
StatePublished - Mar 2017

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Intestinal Neoplasms
modifiers (genes)
carcinogenesis
Carcinogenesis
neoplasms
Proteins
proteins
mice
Tumors
Alleles
alleles
colorectal neoplasms
Colorectal Neoplasms
germ cells
Neoplasms
heterozygosity
Hereditary Nonpolyposis Colorectal Neoplasms
centrosomes
Transport Vesicles
somatic mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Evaluation of Rint1 as a modifier of intestinal tumorigenesis and cancer risk. / Otterpohl, Karla L.; Gould, Karen A.

In: PloS one, Vol. 12, No. 3, e0172247, 03.2017.

Research output: Contribution to journalArticle

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