Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus

B. Pattnaik, A. Sanyal, Manju George, C. Tosh, D. Hemadri, R. Venkataramanan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1393 bp and the smallest was of 208 bp in size. The 1393 bp fragment included sequences from the preceeding P18 region of FMDV genome. The second largest fragment of 734 bp included sequences from the 3'-extracistronic region of viral genome. The remaining fragments were amplified either from the 3'- or 5'-half of the 3D gene. Specific amplification of the entire 3D gene in fragments of different size showed sequence conservation in the 3D genomic region of FMDV and usefulness of the primers reported in detecting inapparent or persistent FMDV infection in susceptible animals by RT-PCR.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalActa Virologica
Volume41
Issue number6
StatePublished - Dec 1 1997

Fingerprint

Foot-and-Mouth Disease Virus
DNA-Directed RNA Polymerases
Polymerase Chain Reaction
Genes
Reverse Transcription
DNA Primers
Viral Genome
Virus Diseases
Genome

Keywords

  • 3D gene
  • Foot-and-mouth disease virus
  • Persistent infection
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Pattnaik, B., Sanyal, A., George, M., Tosh, C., Hemadri, D., & Venkataramanan, R. (1997). Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus. Acta Virologica, 41(6), 333-336.

Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus. / Pattnaik, B.; Sanyal, A.; George, Manju; Tosh, C.; Hemadri, D.; Venkataramanan, R.

In: Acta Virologica, Vol. 41, No. 6, 01.12.1997, p. 333-336.

Research output: Contribution to journalArticle

Pattnaik, B, Sanyal, A, George, M, Tosh, C, Hemadri, D & Venkataramanan, R 1997, 'Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus', Acta Virologica, vol. 41, no. 6, pp. 333-336.
Pattnaik, B. ; Sanyal, A. ; George, Manju ; Tosh, C. ; Hemadri, D. ; Venkataramanan, R. / Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus. In: Acta Virologica. 1997 ; Vol. 41, No. 6. pp. 333-336.
@article{11cce110b8584fe2be7e641b28f3e7c9,
title = "Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus",
abstract = "Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1393 bp and the smallest was of 208 bp in size. The 1393 bp fragment included sequences from the preceeding P18 region of FMDV genome. The second largest fragment of 734 bp included sequences from the 3'-extracistronic region of viral genome. The remaining fragments were amplified either from the 3'- or 5'-half of the 3D gene. Specific amplification of the entire 3D gene in fragments of different size showed sequence conservation in the 3D genomic region of FMDV and usefulness of the primers reported in detecting inapparent or persistent FMDV infection in susceptible animals by RT-PCR.",
keywords = "3D gene, Foot-and-mouth disease virus, Persistent infection, Reverse transcription-polymerase chain reaction",
author = "B. Pattnaik and A. Sanyal and Manju George and C. Tosh and D. Hemadri and R. Venkataramanan",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "41",
pages = "333--336",
journal = "Acta Virologica",
issn = "0001-723X",
publisher = "AEP - Academic Electronic Press Ltd.",
number = "6",

}

TY - JOUR

T1 - Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus

AU - Pattnaik, B.

AU - Sanyal, A.

AU - George, Manju

AU - Tosh, C.

AU - Hemadri, D.

AU - Venkataramanan, R.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1393 bp and the smallest was of 208 bp in size. The 1393 bp fragment included sequences from the preceeding P18 region of FMDV genome. The second largest fragment of 734 bp included sequences from the 3'-extracistronic region of viral genome. The remaining fragments were amplified either from the 3'- or 5'-half of the 3D gene. Specific amplification of the entire 3D gene in fragments of different size showed sequence conservation in the 3D genomic region of FMDV and usefulness of the primers reported in detecting inapparent or persistent FMDV infection in susceptible animals by RT-PCR.

AB - Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1393 bp and the smallest was of 208 bp in size. The 1393 bp fragment included sequences from the preceeding P18 region of FMDV genome. The second largest fragment of 734 bp included sequences from the 3'-extracistronic region of viral genome. The remaining fragments were amplified either from the 3'- or 5'-half of the 3D gene. Specific amplification of the entire 3D gene in fragments of different size showed sequence conservation in the 3D genomic region of FMDV and usefulness of the primers reported in detecting inapparent or persistent FMDV infection in susceptible animals by RT-PCR.

KW - 3D gene

KW - Foot-and-mouth disease virus

KW - Persistent infection

KW - Reverse transcription-polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0031405839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031405839&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 333

EP - 336

JO - Acta Virologica

JF - Acta Virologica

SN - 0001-723X

IS - 6

ER -