Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

A. S. Zhuk, E. I. Stepchenkova, Youri I Pavlov, S. G. Inge-Vechtomov

Research output: Contribution to journalArticle

Abstract

Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

Original languageEnglish (US)
Pages (from-to)111-122
Number of pages12
JournalCell and Tissue Biology
Volume11
Issue number2
DOIs
StatePublished - Mar 1 2017

Fingerprint

Cell Division
Saccharomyces cerevisiae
Yeasts
Cell Cycle
Biochemical Phenomena
Nocodazole
Hydroxyurea
Gene Expression Regulation
Mutation
Temperature

Keywords

  • alpha-factor
  • cdc28-4
  • flow cytometry
  • hydroxyurea
  • nocodazole
  • synchronize of cell division
  • yeast Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Cell Biology

Cite this

Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae. / Zhuk, A. S.; Stepchenkova, E. I.; Pavlov, Youri I; Inge-Vechtomov, S. G.

In: Cell and Tissue Biology, Vol. 11, No. 2, 01.03.2017, p. 111-122.

Research output: Contribution to journalArticle

Zhuk, A. S. ; Stepchenkova, E. I. ; Pavlov, Youri I ; Inge-Vechtomov, S. G. / Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae. In: Cell and Tissue Biology. 2017 ; Vol. 11, No. 2. pp. 111-122.
@article{cf05ec5edc9749dfa75ec2b9315628aa,
title = "Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae",
abstract = "Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).",
keywords = "alpha-factor, cdc28-4, flow cytometry, hydroxyurea, nocodazole, synchronize of cell division, yeast Saccharomyces cerevisiae",
author = "Zhuk, {A. S.} and Stepchenkova, {E. I.} and Pavlov, {Youri I} and Inge-Vechtomov, {S. G.}",
year = "2017",
month = "3",
day = "1",
doi = "10.1134/S1990519X17020110",
language = "English (US)",
volume = "11",
pages = "111--122",
journal = "Cell and Tissue Biology",
issn = "1990-519X",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "2",

}

TY - JOUR

T1 - Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

AU - Zhuk, A. S.

AU - Stepchenkova, E. I.

AU - Pavlov, Youri I

AU - Inge-Vechtomov, S. G.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

AB - Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

KW - alpha-factor

KW - cdc28-4

KW - flow cytometry

KW - hydroxyurea

KW - nocodazole

KW - synchronize of cell division

KW - yeast Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=85018499854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018499854&partnerID=8YFLogxK

U2 - 10.1134/S1990519X17020110

DO - 10.1134/S1990519X17020110

M3 - Article

AN - SCOPUS:85018499854

VL - 11

SP - 111

EP - 122

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 2

ER -