Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia

Kuldeep S. Attri, Kamiya Mehla, Pankaj Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Hypoxic microenvironment found in pancreatic ductal adenocarcinoma and other solid tumors is central to physiological and metabolic alterations of immune cells that significantly impact tumor growth dynamics. Hypoxic adaptations in the immune cells are primarily mediated by the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which regulates cellular metabolism by modulating glycolysis and other interconnected metabolic pathways. HIF-1α plays distinct roles in M1 and M2 macrophage polarization, which, in turn, regulates tumor cell immune escape and growth. In this chapter, we describe a real-time PCR-based assay to monitor the transcript levels of Arg1 and Nos2 to assess the status of tumor-induced macrophage polarization under hypoxic conditions. This method can be effectively utilized to delineate the genes critical for M1/M2 polarization in the hypoxic tumor microenvironment and would provide opportunities to develop immunomodulating therapies to regulate the tumor growth, progression, and metastatic dissemination.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages265-276
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Fingerprint

Tumor Microenvironment
Pancreatic Neoplasms
Macrophages
Hypoxia-Inducible Factor 1
Neoplasms
Growth
Tumor Escape
Glycolysis
Metabolic Networks and Pathways
Real-Time Polymerase Chain Reaction
Adenocarcinoma
Hypoxia
Genes
Therapeutics

Keywords

  • HIF-1α
  • Hypoxia
  • Macrophages
  • Metabolism
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Attri, K. S., Mehla, K., & Singh, P. (2018). Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia. In Methods in Molecular Biology (pp. 265-276). (Methods in Molecular Biology; Vol. 1742). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7665-2_23

Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia. / Attri, Kuldeep S.; Mehla, Kamiya; Singh, Pankaj.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 265-276 (Methods in Molecular Biology; Vol. 1742).

Research output: Chapter in Book/Report/Conference proceedingChapter

Attri, KS, Mehla, K & Singh, P 2018, Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1742, Humana Press Inc., pp. 265-276. https://doi.org/10.1007/978-1-4939-7665-2_23
Attri KS, Mehla K, Singh P. Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 265-276. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7665-2_23
Attri, Kuldeep S. ; Mehla, Kamiya ; Singh, Pankaj. / Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 265-276 (Methods in Molecular Biology).
@inbook{564bae6be41b4f648559646e9875c7c4,
title = "Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia",
abstract = "Hypoxic microenvironment found in pancreatic ductal adenocarcinoma and other solid tumors is central to physiological and metabolic alterations of immune cells that significantly impact tumor growth dynamics. Hypoxic adaptations in the immune cells are primarily mediated by the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which regulates cellular metabolism by modulating glycolysis and other interconnected metabolic pathways. HIF-1α plays distinct roles in M1 and M2 macrophage polarization, which, in turn, regulates tumor cell immune escape and growth. In this chapter, we describe a real-time PCR-based assay to monitor the transcript levels of Arg1 and Nos2 to assess the status of tumor-induced macrophage polarization under hypoxic conditions. This method can be effectively utilized to delineate the genes critical for M1/M2 polarization in the hypoxic tumor microenvironment and would provide opportunities to develop immunomodulating therapies to regulate the tumor growth, progression, and metastatic dissemination.",
keywords = "HIF-1α, Hypoxia, Macrophages, Metabolism, Pancreatic cancer",
author = "Attri, {Kuldeep S.} and Kamiya Mehla and Pankaj Singh",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7665-2_23",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "265--276",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Evaluation of macrophage polarization in pancreatic cancer microenvironment under hypoxia

AU - Attri, Kuldeep S.

AU - Mehla, Kamiya

AU - Singh, Pankaj

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Hypoxic microenvironment found in pancreatic ductal adenocarcinoma and other solid tumors is central to physiological and metabolic alterations of immune cells that significantly impact tumor growth dynamics. Hypoxic adaptations in the immune cells are primarily mediated by the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which regulates cellular metabolism by modulating glycolysis and other interconnected metabolic pathways. HIF-1α plays distinct roles in M1 and M2 macrophage polarization, which, in turn, regulates tumor cell immune escape and growth. In this chapter, we describe a real-time PCR-based assay to monitor the transcript levels of Arg1 and Nos2 to assess the status of tumor-induced macrophage polarization under hypoxic conditions. This method can be effectively utilized to delineate the genes critical for M1/M2 polarization in the hypoxic tumor microenvironment and would provide opportunities to develop immunomodulating therapies to regulate the tumor growth, progression, and metastatic dissemination.

AB - Hypoxic microenvironment found in pancreatic ductal adenocarcinoma and other solid tumors is central to physiological and metabolic alterations of immune cells that significantly impact tumor growth dynamics. Hypoxic adaptations in the immune cells are primarily mediated by the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which regulates cellular metabolism by modulating glycolysis and other interconnected metabolic pathways. HIF-1α plays distinct roles in M1 and M2 macrophage polarization, which, in turn, regulates tumor cell immune escape and growth. In this chapter, we describe a real-time PCR-based assay to monitor the transcript levels of Arg1 and Nos2 to assess the status of tumor-induced macrophage polarization under hypoxic conditions. This method can be effectively utilized to delineate the genes critical for M1/M2 polarization in the hypoxic tumor microenvironment and would provide opportunities to develop immunomodulating therapies to regulate the tumor growth, progression, and metastatic dissemination.

KW - HIF-1α

KW - Hypoxia

KW - Macrophages

KW - Metabolism

KW - Pancreatic cancer

UR - http://www.scopus.com/inward/record.url?scp=85040867568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040867568&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7665-2_23

DO - 10.1007/978-1-4939-7665-2_23

M3 - Chapter

C2 - 29330807

AN - SCOPUS:85040867568

T3 - Methods in Molecular Biology

SP - 265

EP - 276

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -