Evaluation of kinetic parameters of a sulfur-limestone autotrophic denitrification biofilm process

Hui Zeng, Tian C. Zhang

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

In this study, four kinetic parameters of autotrophic denitrifiers in fixed-bed sulfur-limestone autotrophic denitrification (SLAD) columns were evaluated. The curve-matching method was used by conducting 22 non-steady-state tests for estimation of half-velocity constant, Ks and maximum specific substrate utilization rate, k. To estimate the bacteria yield coefficient, Y and the decay coefficient, kd, two short term batch tests (before and after the starvation of the autotrophic denitrifiers) were conducted using a fixed-bed SLAD column where the biofilm was fully penetrated by nitrate-N. It was found that Ks=0.398 mg NO3 --N/l, k=0.15 d-1, kd=0.09-0.12 d-1, and Y=0.85-1.11 g VSS/g NO3--N. Our results are consistent with those obtained from SLAD biofilm processes, but different from those obtained from suspended-growth systems with thiosulfate or sulfur powders as the S source. The method developed in this study might be useful for estimation of four Monod-type kinetic parameters in other biofilm processes. However, cautions must be given when the estimated parameters are used because the measurements of the biomass and the biofilm thickness could be further improved, and the assumption of sulfur being a non-limiting substrate needs to be proved.

Original languageEnglish (US)
Pages (from-to)4941-4952
Number of pages12
JournalWater Research
Volume39
Issue number20
DOIs
StatePublished - Dec 2005

Fingerprint

Denitrification
Biofilms
Limestone
Kinetic parameters
biofilm
denitrification
Sulfur
sulfur
limestone
kinetics
substrate
thiosulfate
Substrates
starvation
Nitrates
Bacteria
Biomass
evaluation
parameter
nitrate

Keywords

  • Autotrophic denitrification
  • Biofilm
  • Kinetics
  • Nitrate
  • Sulfur

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Evaluation of kinetic parameters of a sulfur-limestone autotrophic denitrification biofilm process. / Zeng, Hui; Zhang, Tian C.

In: Water Research, Vol. 39, No. 20, 12.2005, p. 4941-4952.

Research output: Contribution to journalArticle

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