Evaluation of immunologic and intestinal effects in rats administered an E 171-containing diet, a food grade titanium dioxide (TiO2)

Lance K. Blevins, Robert B. Crawford, Anthony Bach, Michael D. Rizzo, Jiajun Zhou, Joseph E. Henriquez, D. M.Isha Olive Khan, Sera Sermet, Lora L Arnold, Karen L. Pennington, Nathalia P. Souza, Samuel Monroe Cohen, Norbert E. Kaminski

Research output: Contribution to journalArticle

Abstract

The toxicity of dietary E 171, a food grade titanium dioxide was evaluated. A recent study reported rats receiving E 171 in water developed inflammation and aberrant crypt foci (ACF) in the gastrointestinal tract. Here, rats received food containing E 171 (7 or 100 days). The 100-day study included feeding E 171 after dimethylhydrazine (DMH) or vehicle only pretreatment. Food consumption was similar between treatment groups with maximum total cumulative E 171 exposure being 2617 mg/kg in 7 days and 29,400 mg/kg in 100 days. No differences were observed due to E 171 in the percentage of dendritic, CD4+ T or Treg cells within Peyer's patches or the periphery, or in cytokine production in plasma, sections of jejunum, and colon in 7- or 100-day E 171 alone fed rats. Differences were observed for IL-17A in colon (400 ppm E 171 + DMH) and IL-12p70 in plasma (40 ppm E 171 + DMH). E 171 had no effect on histopathologic evaluations of small and large intestines, liver, spleen, lungs, or testes, and no effects on ACF, goblet cell numbers, or colonic gland length. Dietary E 171 administration (7- or 100-day), even at high doses, produced no effect on the immune parameters or tissue morphology.

Original languageEnglish (US)
Article number110793
JournalFood and Chemical Toxicology
Volume133
DOIs
StatePublished - Nov 1 2019

Fingerprint

Dimethylhydrazines
titanium dioxide
food grades
Nutrition
Aberrant Crypt Foci
Rats
Diet
Food
colon
rats
Colon
diet
Plasmas
Peyer's Patches
Peyer's patches
Goblet Cells
goblet cells
Interleukin-17
Large Intestine
large intestine

Keywords

  • Dietary titanium dioxide
  • E 171
  • Gastrointestinal inflammation
  • Intestinal carcinogenesis
  • T cells

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Evaluation of immunologic and intestinal effects in rats administered an E 171-containing diet, a food grade titanium dioxide (TiO2). / Blevins, Lance K.; Crawford, Robert B.; Bach, Anthony; Rizzo, Michael D.; Zhou, Jiajun; Henriquez, Joseph E.; Khan, D. M.Isha Olive; Sermet, Sera; Arnold, Lora L; Pennington, Karen L.; Souza, Nathalia P.; Cohen, Samuel Monroe; Kaminski, Norbert E.

In: Food and Chemical Toxicology, Vol. 133, 110793, 01.11.2019.

Research output: Contribution to journalArticle

Blevins, LK, Crawford, RB, Bach, A, Rizzo, MD, Zhou, J, Henriquez, JE, Khan, DMIO, Sermet, S, Arnold, LL, Pennington, KL, Souza, NP, Cohen, SM & Kaminski, NE 2019, 'Evaluation of immunologic and intestinal effects in rats administered an E 171-containing diet, a food grade titanium dioxide (TiO2)', Food and Chemical Toxicology, vol. 133, 110793. https://doi.org/10.1016/j.fct.2019.110793
Blevins, Lance K. ; Crawford, Robert B. ; Bach, Anthony ; Rizzo, Michael D. ; Zhou, Jiajun ; Henriquez, Joseph E. ; Khan, D. M.Isha Olive ; Sermet, Sera ; Arnold, Lora L ; Pennington, Karen L. ; Souza, Nathalia P. ; Cohen, Samuel Monroe ; Kaminski, Norbert E. / Evaluation of immunologic and intestinal effects in rats administered an E 171-containing diet, a food grade titanium dioxide (TiO2). In: Food and Chemical Toxicology. 2019 ; Vol. 133.
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abstract = "The toxicity of dietary E 171, a food grade titanium dioxide was evaluated. A recent study reported rats receiving E 171 in water developed inflammation and aberrant crypt foci (ACF) in the gastrointestinal tract. Here, rats received food containing E 171 (7 or 100 days). The 100-day study included feeding E 171 after dimethylhydrazine (DMH) or vehicle only pretreatment. Food consumption was similar between treatment groups with maximum total cumulative E 171 exposure being 2617 mg/kg in 7 days and 29,400 mg/kg in 100 days. No differences were observed due to E 171 in the percentage of dendritic, CD4+ T or Treg cells within Peyer's patches or the periphery, or in cytokine production in plasma, sections of jejunum, and colon in 7- or 100-day E 171 alone fed rats. Differences were observed for IL-17A in colon (400 ppm E 171 + DMH) and IL-12p70 in plasma (40 ppm E 171 + DMH). E 171 had no effect on histopathologic evaluations of small and large intestines, liver, spleen, lungs, or testes, and no effects on ACF, goblet cell numbers, or colonic gland length. Dietary E 171 administration (7- or 100-day), even at high doses, produced no effect on the immune parameters or tissue morphology.",
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AU - Henriquez, Joseph E.

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