Evaluation of hypothalamic gene expression in mice divergently selected for heat loss

Stephanie R. Wesolowski, Mark F. Allan, Merlyn K Nielsen, Daniel Pomp

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mouse lines divergently selected for heat loss were evaluated for correlated responses in the hypothalamic transcriptome. High (MH) heat loss mice have ∼50% greater heat loss, ∼35% less body fat, ∼20% greater feed intake, ∼100% greater locomotor activity levels, and higher core body temperature compared with low (ML) heat loss mice. We evaluated hypothalamic expression between inbred lines derived from MH and ML lines (IH and IL, respectively) using cDNA microarrays and selected genes previously isolated in a large differential-display PCR experiment. Northern analysis was used to confirm differences, revealing higher hypothalamic mRNA expression of oxytocin (Oxt) and tissue inhibitor of metalloproteinase 2 (Timp-2) in the IH line. Real-time PCR assays were developed for Oxt, Timp-2, and ribosomal protein L3 (Rpl3, previously found to be upregulated in IL) and confirmed differential expression of these genes with potential physiological relevance in energy balance. These results provide information on correlated responses in the transcriptome of mice selected for high and low energy expenditure and reveal new information regarding genetic regulation of energy balance.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalPhysiological genomics
Volume13
DOIs
StatePublished - Jan 1 2003

Fingerprint

Hot Temperature
Gene Expression
Tissue Inhibitor of Metalloproteinase-2
Oxytocin
Transcriptome
Locomotion
Oligonucleotide Array Sequence Analysis
Body Temperature
Energy Metabolism
Adipose Tissue
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Messenger RNA
Genes

Keywords

  • Microarrays
  • Obesity
  • Real-time polymerase chain reaction

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Evaluation of hypothalamic gene expression in mice divergently selected for heat loss. / Wesolowski, Stephanie R.; Allan, Mark F.; Nielsen, Merlyn K; Pomp, Daniel.

In: Physiological genomics, Vol. 13, 01.01.2003, p. 129-137.

Research output: Contribution to journalArticle

Wesolowski, Stephanie R. ; Allan, Mark F. ; Nielsen, Merlyn K ; Pomp, Daniel. / Evaluation of hypothalamic gene expression in mice divergently selected for heat loss. In: Physiological genomics. 2003 ; Vol. 13. pp. 129-137.
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