Evaluation of higher plant virus resistance genes in the green alga, Chlorella variabilis NC64A, during the early phase of infection with Paramecium bursaria chlorella virus-1

Janet M. Rowe, David D. Dunigan, Guillaume Blanc, James R. Gurnon, Yuannan Xia, James L. Van Etten

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

With growing industrial interest in algae plus their critical roles in aquatic systems, the need to understand the effects of algal pathogens is increasing. We examined a model algal host-virus system, Chlorella variabilis NC64A and virus, PBCV-1. C. variabilis encodes 375 homologs to genes involved in RNA silencing and in response to virus infection in higher plants. Illumina RNA-Seq data showed that 325 of these homologs were expressed in healthy and early PBCV-1 infected (≤60. min) cells. For each of the RNA silencing genes to which homologs were found, mRNA transcripts were detected in healthy and infected cells. C. variabilis, like higher plants, may employ certain RNA silencing pathways to defend itself against virus infection. To our knowledge this is the first examination of RNA silencing genes in algae beyond core proteins, and the first analysis of their transcription during virus infection.

Original languageEnglish (US)
Pages (from-to)101-113
Number of pages13
JournalVirology
Volume442
Issue number2
DOIs
StatePublished - Aug 1 2013

Fingerprint

Paramecium
Chlorella
Plant Viruses
Chlorophyta
RNA Interference
Virus Diseases
Viruses
Infection
Genes
Phycodnaviridae
RNA
Messenger RNA
Proteins

Keywords

  • Phycodnaviridae
  • Phytoplankton
  • Post-transcriptional gene silencing
  • RNA directed DNA methylation
  • RNA silencing
  • Transcriptome

ASJC Scopus subject areas

  • Virology

Cite this

Evaluation of higher plant virus resistance genes in the green alga, Chlorella variabilis NC64A, during the early phase of infection with Paramecium bursaria chlorella virus-1. / Rowe, Janet M.; Dunigan, David D.; Blanc, Guillaume; Gurnon, James R.; Xia, Yuannan; Van Etten, James L.

In: Virology, Vol. 442, No. 2, 01.08.2013, p. 101-113.

Research output: Contribution to journalArticle

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