Evaluation of Expression Profiles of Hematopoietic Stem Cell, Endothelial Cell, and Myeloid Cell Antigens in Spontaneous and Chemically Induced Hemangiosarcomas and Hemangiomas in Mice

Satoko Kakiuchi-Kiyota, Torrie A. Crabbs, Lora L Arnold, Karen L. Pennington, Jon C. Cook, David E. Malarkey, Samuel Monroe Cohen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It is unclear whether the process of spontaneous and chemically induced hemangiosarcoma and hemangioma formation in mice involves the transformation of differentiated endothelial cells (ECs) or recruitment of multipotential bone marrow–derived hematopoietic stem cells or endothelial progenitor cells (EPCs), which show some degree of endothelial differentiation. In the present study, immunohistochemical staining for hematopoietic stem cell markers (CD45 and CD34), EC markers (vascular endothelial growth factor receptor 2 [VEGFR2], CD31, and factor VIII–related antigen), and a myeloid lineage marker (CD14) was employed to better define the origin of hemangiosarcomas and hemangiomas in mice. Staining was negative for CD45, factor VIII–related antigen, and CD14 and positive for CD34, VEGFR2, and CD31, indicating that mouse hemangiosarcomas and hemangiomas are composed of cells derived from EPCs expressing CD34, VEGFR2, and CD31 but not factor VIII–related antigen. The lack of CD45 expression suggests that mouse vascular tumors may arise from EPCs that are at a stage later than hematopoietic stem cells. Since factor VIII–related antigen expression is known to occur later than CD31 expression in EPCs, our observations may indicate that these tumor cells are arrested at a stage prior to complete differentiation.  In addition, myeloid lineage cells do not appear to contribute to hemangiosarcoma and hemangioma formation in mice.

Original languageEnglish (US)
Pages (from-to)709-721
Number of pages13
JournalToxicologic Pathology
Volume41
Issue number5
DOIs
StatePublished - Jul 2013

Fingerprint

Hemangiosarcoma
Endothelial cells
Myeloid Cells
Hemangioma
Hematopoietic Stem Cells
Stem cells
Endothelial Cells
Vascular Endothelial Growth Factor Receptor-2
Antigens
Tumors
CD14 Antigens
Negative Staining
Blood Vessels
Neoplasms
Staining and Labeling
Bone
Bone and Bones
Cells
Endothelial Progenitor Cells

Keywords

  • endothelial progenitor cells
  • hemangiomas
  • hemangiosarcomas
  • hematopoietic stem cells
  • immunohistochemistry
  • mouse

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Evaluation of Expression Profiles of Hematopoietic Stem Cell, Endothelial Cell, and Myeloid Cell Antigens in Spontaneous and Chemically Induced Hemangiosarcomas and Hemangiomas in Mice. / Kakiuchi-Kiyota, Satoko; Crabbs, Torrie A.; Arnold, Lora L; Pennington, Karen L.; Cook, Jon C.; Malarkey, David E.; Cohen, Samuel Monroe.

In: Toxicologic Pathology, Vol. 41, No. 5, 07.2013, p. 709-721.

Research output: Contribution to journalArticle

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