Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution

Deepta A. Ghate, Glenn Holley, Harli Dollinger, Joseph P. Bullock, Kerry Markwardt, Henry F. Edelhauser

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS. Methods: Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80% power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50% power, 50% of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints. Results: The mucin layer thickness in the continuous phaco group was 0.77 ± 0.02 μm (mean ± SE) with NGOIS and 0.51 ± 0.01 μm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 ± 0.02 μm with NGOIS and 0.54 ± 0.01 μm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 ± 0.02 μm. The endothelial ultrastructure was normal in all corneas. Conclusions: In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US).

Original languageEnglish (US)
Pages (from-to)1050-1056
Number of pages7
JournalCornea
Volume27
Issue number9
DOIs
StatePublished - Oct 1 2008

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Phacoemulsification
Ophthalmic Solutions
Mucins
Cornea
Transmission Electron Microscopy
Control Groups
Surface Tension
Anterior Chamber
Viscosity
Staining and Labeling

Keywords

  • Endothelial mucin layer
  • Irrigating solution
  • Phacoemulsification

ASJC Scopus subject areas

  • Medicine(all)
  • Ophthalmology

Cite this

Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution. / Ghate, Deepta A.; Holley, Glenn; Dollinger, Harli; Bullock, Joseph P.; Markwardt, Kerry; Edelhauser, Henry F.

In: Cornea, Vol. 27, No. 9, 01.10.2008, p. 1050-1056.

Research output: Contribution to journalArticle

Ghate, Deepta A. ; Holley, Glenn ; Dollinger, Harli ; Bullock, Joseph P. ; Markwardt, Kerry ; Edelhauser, Henry F. / Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution. In: Cornea. 2008 ; Vol. 27, No. 9. pp. 1050-1056.
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abstract = "Purpose: To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS. Methods: Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80{\%} power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50{\%} power, 50{\%} of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints. Results: The mucin layer thickness in the continuous phaco group was 0.77 ± 0.02 μm (mean ± SE) with NGOIS and 0.51 ± 0.01 μm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 ± 0.02 μm with NGOIS and 0.54 ± 0.01 μm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 ± 0.02 μm. The endothelial ultrastructure was normal in all corneas. Conclusions: In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US).",
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T1 - Evaluation of endothelial mucin layer thickness after phacoemulsification with next generation ophthalmic irrigating solution

AU - Ghate, Deepta A.

AU - Holley, Glenn

AU - Dollinger, Harli

AU - Bullock, Joseph P.

AU - Markwardt, Kerry

AU - Edelhauser, Henry F.

PY - 2008/10/1

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N2 - Purpose: To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS. Methods: Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80% power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50% power, 50% of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints. Results: The mucin layer thickness in the continuous phaco group was 0.77 ± 0.02 μm (mean ± SE) with NGOIS and 0.51 ± 0.01 μm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 ± 0.02 μm with NGOIS and 0.54 ± 0.01 μm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 ± 0.02 μm. The endothelial ultrastructure was normal in all corneas. Conclusions: In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US).

AB - Purpose: To evaluate human corneal endothelial mucin layer thickness and ultrastructure after phacoemulsification and irrigation-aspiration with either next generation ophthalmic irrigating solution (NGOIS) or BSS PLUS. Methods: Paired human corneas were mounted in an artificial anterior chamber, exposed to 3 minutes of continuous ultrasound (US) at 80% power using the Alcon SERIES 20000 LEGACY surgical system (n = 9) or to 2 minutes of pulsed US at 50% power, 50% of the time at 20 pps using the Alcon INFINITI Vision System (n = 5), and irrigated with 250 mL of either NGOIS or BSS PLUS. A control group of paired corneas did not undergo phacoemulsification or irrigation-aspiration (n = 5). Corneas were divided and fixed for mucin staining or transmission electron microscopy. Mucin layer thickness was measured on the transmission electron microscopy prints. Results: The mucin layer thickness in the continuous phaco group was 0.77 ± 0.02 μm (mean ± SE) with NGOIS and 0.51 ± 0.01 μm with BSS PLUS (t test, P < 0.001). The mucin layer thickness in the pulsed phaco group was 0.79 ± 0.02 μm with NGOIS and 0.54 ± 0.01 μm with BSS PLUS (P < 0.001). The mucin layer thickness in the untreated control group was 0.72 ± 0.02 μm. The endothelial ultrastructure was normal in all corneas. Conclusions: In this in vitro corneal model, NGOIS, due to its lower surface tension and higher viscosity, preserved endothelial mucin layer thickness better than BSS PLUS with both the INFINITI Vision System (pulsed US) and the LEGACY surgical system (continuous US).

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KW - Irrigating solution

KW - Phacoemulsification

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