Evaluation of effects of chitosan in preventing hemorrhagic cystitis in rats induced by cyclophosphamide

T. Okamura, T. Masui, M. K. St. John, S. M. Cohen, R. J. Taylor

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hemorrhagic cystitis is a common problem following cyclophosphamide or radiation therapy. Chitosan has been shown to be an effective hemostatic agent and promoter of wound healing in animal experiments. We evaluated the safety and efficacy of intravesical chitosan in an animal model of cyclophosphamide cystitis. Hemorrhagic cystitis was induced in female E 344 rats by intraperitoneal cyclophosphamide, 100 mg/kg. Chitosan solution (0.3 ml) was instilled intravesically on day 1 (Group 1), on days 1, 3, and 5 (Group 2), or 1 hour after the administration of cyclophosphamide (Group 3). The rats in group 4 were treated with chitosan diluent on day 1 after cyclophosphamide, and the rats in group 5 received intravesical chitosan without cyclophosphamide. Sequential examination revealed decreased mortality and lower incidences of severe bladder bleeding, necrosis and inflammation in Group 3. Treatment delayed until after the appearance of the cystitis, especially repeated treatments, appeared to make the cyclophosphamide-induced changes worse. Used within 1 hour of cyclophosphamide administration, before the cystitis develops, chitosan seemed to have the possibility to inhibit the appearance of hemorrhagic cystitis. In addition to the changes in the bladder, severe changes occurred in the kidneys secondary to cyclophosphamide.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalActa Urologica Japonica
Volume41
Issue number4
StatePublished - Jan 1 1995

Fingerprint

Cystitis
Chitosan
Cyclophosphamide
Urinary Bladder
Hemostatics
Wound Healing
Necrosis
Radiotherapy
Animal Models
Hemorrhage
Inflammation
Kidney
Safety
Mortality
Incidence

Keywords

  • bladder instillation
  • chitosan
  • cyclophosphamide
  • hemorrhagic cystitis

ASJC Scopus subject areas

  • Urology

Cite this

Evaluation of effects of chitosan in preventing hemorrhagic cystitis in rats induced by cyclophosphamide. / Okamura, T.; Masui, T.; St. John, M. K.; Cohen, S. M.; Taylor, R. J.

In: Acta Urologica Japonica, Vol. 41, No. 4, 01.01.1995, p. 289-296.

Research output: Contribution to journalArticle

Okamura, T. ; Masui, T. ; St. John, M. K. ; Cohen, S. M. ; Taylor, R. J. / Evaluation of effects of chitosan in preventing hemorrhagic cystitis in rats induced by cyclophosphamide. In: Acta Urologica Japonica. 1995 ; Vol. 41, No. 4. pp. 289-296.
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