Evaluation of an immobilized-enzyme analyzer for measuring galactose in serum

G. J. Buffone, J. M. Johnson, S. A. Lewis, J. W. Sparks

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Reportedly, galactose provides an alternative carbohydrate source and improved homeostatic regulation of glucose in the premature infant. Because of its potentially toxic effects, sensitive methods are needed for monitoring its concentration during therapy. The authors evaluated an immobilized galactose oxidase/hydrogen peroxide electrode system and a modified homogeneous enzymic method. Both methods are suitable for measuring galactose in a small sample and are comparably precise. The latter method gives superior analytical recoveries below 100 mg/L, but is linear in absorbance response to only 300 mg/L. It was found that the immobilized-enzyme method superior for monitoring treatment of neonates with galactose, because it requires only a few minutes and 25 μL of serum, and the analytical procedure is simpler.

Original languageEnglish (US)
Pages (from-to)339-340
Number of pages2
JournalClinical Chemistry
Volume26
Issue number2
StatePublished - Jan 1 1980

Fingerprint

Immobilized Enzymes
Galactose
Serum
Galactose Oxidase
Monitoring
Poisons
Hydrogen Peroxide
Carbohydrates
Glucose
Recovery
Electrodes
Premature Infants
Newborn Infant
Therapeutics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Evaluation of an immobilized-enzyme analyzer for measuring galactose in serum. / Buffone, G. J.; Johnson, J. M.; Lewis, S. A.; Sparks, J. W.

In: Clinical Chemistry, Vol. 26, No. 2, 01.01.1980, p. 339-340.

Research output: Contribution to journalArticle

Buffone, GJ, Johnson, JM, Lewis, SA & Sparks, JW 1980, 'Evaluation of an immobilized-enzyme analyzer for measuring galactose in serum', Clinical Chemistry, vol. 26, no. 2, pp. 339-340.
Buffone, G. J. ; Johnson, J. M. ; Lewis, S. A. ; Sparks, J. W. / Evaluation of an immobilized-enzyme analyzer for measuring galactose in serum. In: Clinical Chemistry. 1980 ; Vol. 26, No. 2. pp. 339-340.
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