Evaluation of abbott TestPack RSV for the diagnosis of respiratory syncytial virus infections

Margaret A. Olsen, Kathleen M. Shuck, Anthony R. Sambol

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Abbott TestPack RSV, a 20-minute enzyme immunoassay, is available for the rapid diagnosis of respiratory syncytial virus (RSV) infections. We have compared TestPack with a "gold standard" method of virus isolation in traditional tube cultures and shell vials to determine the sensitivity and specificity of this rapid method. Respiratory specimens were collected prospectively from 402 children and assayed by the rapid antigen detection method and isolation in culture. Virus was isolated by inculation of specimen in a total of eight tubes and 2-3 shell vials. Isolation of RSV was confirmed by characteristic cytopathic effect and immunofluorescence using monoclonal antibodies to RSV. Of the 402 specimens tested, there were only 18 discrepant results (seven TestPack-positive, culture-negative, and 11 TestPack-negative, culture-positive specimens). The sensitivity of TestPack RSV versus culture was 93.6% (162 of 173) and the specificity was 97.0% (222 of 229). Using a very rigorous culture system, we have obtained high values for the sensitivity and specificity of TestPack RSV. This assay is an excellent method for the rapid diagnosis of RSV infections in young children.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume16
Issue number2
DOIs
StatePublished - Feb 1993

Fingerprint

Respiratory Syncytial Virus Infections
Respiratory Syncytial Viruses
Viruses
Sensitivity and Specificity
Immunoenzyme Techniques
Fluorescent Antibody Technique
Monoclonal Antibodies
Antigens

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Evaluation of abbott TestPack RSV for the diagnosis of respiratory syncytial virus infections. / Olsen, Margaret A.; Shuck, Kathleen M.; Sambol, Anthony R.

In: Diagnostic Microbiology and Infectious Disease, Vol. 16, No. 2, 02.1993, p. 105-109.

Research output: Contribution to journalArticle

@article{04eae1661f184eccbed25194947d9de4,
title = "Evaluation of abbott TestPack RSV for the diagnosis of respiratory syncytial virus infections",
abstract = "Abbott TestPack RSV, a 20-minute enzyme immunoassay, is available for the rapid diagnosis of respiratory syncytial virus (RSV) infections. We have compared TestPack with a {"}gold standard{"} method of virus isolation in traditional tube cultures and shell vials to determine the sensitivity and specificity of this rapid method. Respiratory specimens were collected prospectively from 402 children and assayed by the rapid antigen detection method and isolation in culture. Virus was isolated by inculation of specimen in a total of eight tubes and 2-3 shell vials. Isolation of RSV was confirmed by characteristic cytopathic effect and immunofluorescence using monoclonal antibodies to RSV. Of the 402 specimens tested, there were only 18 discrepant results (seven TestPack-positive, culture-negative, and 11 TestPack-negative, culture-positive specimens). The sensitivity of TestPack RSV versus culture was 93.6{\%} (162 of 173) and the specificity was 97.0{\%} (222 of 229). Using a very rigorous culture system, we have obtained high values for the sensitivity and specificity of TestPack RSV. This assay is an excellent method for the rapid diagnosis of RSV infections in young children.",
author = "Olsen, {Margaret A.} and Shuck, {Kathleen M.} and Sambol, {Anthony R.}",
year = "1993",
month = "2",
doi = "10.1016/0732-8893(93)90003-P",
language = "English (US)",
volume = "16",
pages = "105--109",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Evaluation of abbott TestPack RSV for the diagnosis of respiratory syncytial virus infections

AU - Olsen, Margaret A.

AU - Shuck, Kathleen M.

AU - Sambol, Anthony R.

PY - 1993/2

Y1 - 1993/2

N2 - Abbott TestPack RSV, a 20-minute enzyme immunoassay, is available for the rapid diagnosis of respiratory syncytial virus (RSV) infections. We have compared TestPack with a "gold standard" method of virus isolation in traditional tube cultures and shell vials to determine the sensitivity and specificity of this rapid method. Respiratory specimens were collected prospectively from 402 children and assayed by the rapid antigen detection method and isolation in culture. Virus was isolated by inculation of specimen in a total of eight tubes and 2-3 shell vials. Isolation of RSV was confirmed by characteristic cytopathic effect and immunofluorescence using monoclonal antibodies to RSV. Of the 402 specimens tested, there were only 18 discrepant results (seven TestPack-positive, culture-negative, and 11 TestPack-negative, culture-positive specimens). The sensitivity of TestPack RSV versus culture was 93.6% (162 of 173) and the specificity was 97.0% (222 of 229). Using a very rigorous culture system, we have obtained high values for the sensitivity and specificity of TestPack RSV. This assay is an excellent method for the rapid diagnosis of RSV infections in young children.

AB - Abbott TestPack RSV, a 20-minute enzyme immunoassay, is available for the rapid diagnosis of respiratory syncytial virus (RSV) infections. We have compared TestPack with a "gold standard" method of virus isolation in traditional tube cultures and shell vials to determine the sensitivity and specificity of this rapid method. Respiratory specimens were collected prospectively from 402 children and assayed by the rapid antigen detection method and isolation in culture. Virus was isolated by inculation of specimen in a total of eight tubes and 2-3 shell vials. Isolation of RSV was confirmed by characteristic cytopathic effect and immunofluorescence using monoclonal antibodies to RSV. Of the 402 specimens tested, there were only 18 discrepant results (seven TestPack-positive, culture-negative, and 11 TestPack-negative, culture-positive specimens). The sensitivity of TestPack RSV versus culture was 93.6% (162 of 173) and the specificity was 97.0% (222 of 229). Using a very rigorous culture system, we have obtained high values for the sensitivity and specificity of TestPack RSV. This assay is an excellent method for the rapid diagnosis of RSV infections in young children.

UR - http://www.scopus.com/inward/record.url?scp=0027469692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027469692&partnerID=8YFLogxK

U2 - 10.1016/0732-8893(93)90003-P

DO - 10.1016/0732-8893(93)90003-P

M3 - Article

C2 - 8467621

AN - SCOPUS:0027469692

VL - 16

SP - 105

EP - 109

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 2

ER -