Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

James L. Bono, James Keen, Laura C. Miller, James M. Fox, Carol G. Chitko-McKown, Michael P. Heaton, William W. Laegreid

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.

Original languageEnglish (US)
Pages (from-to)1855-1857
Number of pages3
JournalApplied and environmental microbiology
Volume70
Issue number3
DOIs
StatePublished - Mar 1 2004

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Escherichia coli O157
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
assay
Erythroid Precursor Cells
cattle
assays
Feces
feces
Sensitivity and Specificity
sampling
testing
evaluation
bovine
rapid test

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples. / Bono, James L.; Keen, James; Miller, Laura C.; Fox, James M.; Chitko-McKown, Carol G.; Heaton, Michael P.; Laegreid, William W.

In: Applied and environmental microbiology, Vol. 70, No. 3, 01.03.2004, p. 1855-1857.

Research output: Contribution to journalArticle

Bono, James L. ; Keen, James ; Miller, Laura C. ; Fox, James M. ; Chitko-McKown, Carol G. ; Heaton, Michael P. ; Laegreid, William W. / Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples. In: Applied and environmental microbiology. 2004 ; Vol. 70, No. 3. pp. 1855-1857.
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