Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum.

P. J. Blackall, Y. Z. Zheng, T. Yamaguchi, Y. Iritani, D. G. Rogers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A panel of four monoclonal antibodies (MAbs) was evaluated, using a hemagglutination-inhibition test, for its ability to subtype 76 isolates of Haemophilus paragallinarum. The results of the MAb reactions were compared with the results of both the Page and Kume serotyping schemes (the serovars of the Page scheme correspond to the serogroups of the Kume scheme). One MAb (E5C12D10) was raised against a Page serovar A strain and the remaining MAbs (F2E6, D6D8D5, and B3E6F9) against a Page serovar C strain. Six different reaction patterns were found among the 76 isolates of H. paragallinarum. There was total correlation between the MAb reaction pattern and the Page scheme, and thus the Kume scheme, to the serogroup level. All 19 Page serovar A (= Kume serogroup A) strains reacted only with MAb E5C12D10, whereas all five Page serovar B (= Kume serogroup B) strains failed to react with any of the MAbs. All 52 remaining strains were Page serovar C (= Kume serogroup C), and all failed to react with MAb E5C12D10 but showed varying reaction patterns with the three other MAbs. Although the MAbs recognized four subdivisions within Kume serogroup C, these subdivisions differed from the four Kume C serovars. This panel of MAbs can be used to assign isolates of H. paragallinarum to either Page serovars or Kume serogroups. Although the subdivisions recognized by the MAbs within the Page serovar C strains do not correspond to the Kume serovars, they may be useful in epidemiological applications.

Original languageEnglish (US)
Pages (from-to)955-959
Number of pages5
JournalAvian diseases
Volume35
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

Haemophilus paragallinarum
Avibacterium paragallinarum
monoclonal antibodies
serotypes
Monoclonal Antibodies
Serogroup

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Blackall, P. J., Zheng, Y. Z., Yamaguchi, T., Iritani, Y., & Rogers, D. G. (1991). Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum. Avian diseases, 35(4), 955-959. https://doi.org/10.2307/1591634

Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum. / Blackall, P. J.; Zheng, Y. Z.; Yamaguchi, T.; Iritani, Y.; Rogers, D. G.

In: Avian diseases, Vol. 35, No. 4, 01.01.1991, p. 955-959.

Research output: Contribution to journalArticle

Blackall, PJ, Zheng, YZ, Yamaguchi, T, Iritani, Y & Rogers, DG 1991, 'Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum.', Avian diseases, vol. 35, no. 4, pp. 955-959. https://doi.org/10.2307/1591634
Blackall, P. J. ; Zheng, Y. Z. ; Yamaguchi, T. ; Iritani, Y. ; Rogers, D. G. / Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum. In: Avian diseases. 1991 ; Vol. 35, No. 4. pp. 955-959.
@article{97f136134686488a936ae1ec001b1ec0,
title = "Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum.",
abstract = "A panel of four monoclonal antibodies (MAbs) was evaluated, using a hemagglutination-inhibition test, for its ability to subtype 76 isolates of Haemophilus paragallinarum. The results of the MAb reactions were compared with the results of both the Page and Kume serotyping schemes (the serovars of the Page scheme correspond to the serogroups of the Kume scheme). One MAb (E5C12D10) was raised against a Page serovar A strain and the remaining MAbs (F2E6, D6D8D5, and B3E6F9) against a Page serovar C strain. Six different reaction patterns were found among the 76 isolates of H. paragallinarum. There was total correlation between the MAb reaction pattern and the Page scheme, and thus the Kume scheme, to the serogroup level. All 19 Page serovar A (= Kume serogroup A) strains reacted only with MAb E5C12D10, whereas all five Page serovar B (= Kume serogroup B) strains failed to react with any of the MAbs. All 52 remaining strains were Page serovar C (= Kume serogroup C), and all failed to react with MAb E5C12D10 but showed varying reaction patterns with the three other MAbs. Although the MAbs recognized four subdivisions within Kume serogroup C, these subdivisions differed from the four Kume C serovars. This panel of MAbs can be used to assign isolates of H. paragallinarum to either Page serovars or Kume serogroups. Although the subdivisions recognized by the MAbs within the Page serovar C strains do not correspond to the Kume serovars, they may be useful in epidemiological applications.",
author = "Blackall, {P. J.} and Zheng, {Y. Z.} and T. Yamaguchi and Y. Iritani and Rogers, {D. G.}",
year = "1991",
month = "1",
day = "1",
doi = "10.2307/1591634",
language = "English (US)",
volume = "35",
pages = "955--959",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "4",

}

TY - JOUR

T1 - Evaluation of a panel of monoclonal antibodies in the subtyping of Haemophilus paragallinarum.

AU - Blackall, P. J.

AU - Zheng, Y. Z.

AU - Yamaguchi, T.

AU - Iritani, Y.

AU - Rogers, D. G.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - A panel of four monoclonal antibodies (MAbs) was evaluated, using a hemagglutination-inhibition test, for its ability to subtype 76 isolates of Haemophilus paragallinarum. The results of the MAb reactions were compared with the results of both the Page and Kume serotyping schemes (the serovars of the Page scheme correspond to the serogroups of the Kume scheme). One MAb (E5C12D10) was raised against a Page serovar A strain and the remaining MAbs (F2E6, D6D8D5, and B3E6F9) against a Page serovar C strain. Six different reaction patterns were found among the 76 isolates of H. paragallinarum. There was total correlation between the MAb reaction pattern and the Page scheme, and thus the Kume scheme, to the serogroup level. All 19 Page serovar A (= Kume serogroup A) strains reacted only with MAb E5C12D10, whereas all five Page serovar B (= Kume serogroup B) strains failed to react with any of the MAbs. All 52 remaining strains were Page serovar C (= Kume serogroup C), and all failed to react with MAb E5C12D10 but showed varying reaction patterns with the three other MAbs. Although the MAbs recognized four subdivisions within Kume serogroup C, these subdivisions differed from the four Kume C serovars. This panel of MAbs can be used to assign isolates of H. paragallinarum to either Page serovars or Kume serogroups. Although the subdivisions recognized by the MAbs within the Page serovar C strains do not correspond to the Kume serovars, they may be useful in epidemiological applications.

AB - A panel of four monoclonal antibodies (MAbs) was evaluated, using a hemagglutination-inhibition test, for its ability to subtype 76 isolates of Haemophilus paragallinarum. The results of the MAb reactions were compared with the results of both the Page and Kume serotyping schemes (the serovars of the Page scheme correspond to the serogroups of the Kume scheme). One MAb (E5C12D10) was raised against a Page serovar A strain and the remaining MAbs (F2E6, D6D8D5, and B3E6F9) against a Page serovar C strain. Six different reaction patterns were found among the 76 isolates of H. paragallinarum. There was total correlation between the MAb reaction pattern and the Page scheme, and thus the Kume scheme, to the serogroup level. All 19 Page serovar A (= Kume serogroup A) strains reacted only with MAb E5C12D10, whereas all five Page serovar B (= Kume serogroup B) strains failed to react with any of the MAbs. All 52 remaining strains were Page serovar C (= Kume serogroup C), and all failed to react with MAb E5C12D10 but showed varying reaction patterns with the three other MAbs. Although the MAbs recognized four subdivisions within Kume serogroup C, these subdivisions differed from the four Kume C serovars. This panel of MAbs can be used to assign isolates of H. paragallinarum to either Page serovars or Kume serogroups. Although the subdivisions recognized by the MAbs within the Page serovar C strains do not correspond to the Kume serovars, they may be useful in epidemiological applications.

UR - http://www.scopus.com/inward/record.url?scp=0026234595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026234595&partnerID=8YFLogxK

U2 - 10.2307/1591634

DO - 10.2307/1591634

M3 - Article

C2 - 1786026

AN - SCOPUS:0026234595

VL - 35

SP - 955

EP - 959

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 4

ER -