4 Citations (Scopus)

Abstract

This study evaluated the performance of a new Food and Drug Administration-approved lateral-flow diagnostic screening test for qualitative detection of West Nile Virus (WNV) immunoglobulin M (IgM) in serum or plasma. Five public health laboratories across the United States performed retrospective testing on blinded serum samples from patients with physician reported diagnoses of WNV infection. The results of the RapidWN™ WNV IgM assay were compared with two commercially available WNV IgM enzyme-linked immunosorbent assays (EIA) and two public health-developed WNV-IgM tests. After discrepancies were resolved, the RapidWN™ WNV IgM EIA demonstrated a 98.8% sensitivity (range: 96.0-100%), a 95.3% specificity (range: 90.9-100%), a positive predictive value of 96.3% (range: 94.7-100%), and a negative predictive value of 98.4% (range: 95.5-100%), as compared to the predicate assays. The study results suggest that the RapidWN™ WNV IgM EIA is an effective, qualitative screening test that produces results comparable to that of predicate assays and can be employed rapidly to detect WNV IgM in patients suspected of having WNV infection.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalJournal of Virological Methods
Volume157
Issue number2
DOIs
StatePublished - May 1 2009

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West Nile virus
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Virus Diseases
Public Health
United States Food and Drug Administration
Serum
Routine Diagnostic Tests
Physicians

Keywords

  • Diagnostic tool
  • Enzyme-linked immunoassay
  • Immunoglobulin M
  • West Nile Virus

ASJC Scopus subject areas

  • Virology

Cite this

Evaluation of a new West Nile Virus lateral-flow rapid IgM assay. / Sambol, Anthony R.; Hinrichs, Steven Heye.

In: Journal of Virological Methods, Vol. 157, No. 2, 01.05.2009, p. 223-226.

Research output: Contribution to journalArticle

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