Ethylammonium nitrate

A protein crystallization reagent

Jennifer A. Garlitz, Catherine A. Summers, Robert A. Flowers, Gloria E Borgstahl

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Ethylammonium nitrate (EAN) is a liquid organic salt that has many potential applications in protein chemistry. Because this solvent has hydrophobic and ionic character as well as the ability to hydrogen bond, it is especially well suited for broad use in protein crystallography. For example, EAN may be used as an additive, a detergent, a precipitating agent or to deliver ligands into protein crystals. A discussion of the crystallization of lysozyme using EAN as a precipitating agent is given here.

Original languageEnglish (US)
Pages (from-to)2037-2038
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number12
DOIs
StatePublished - Dec 1 1999

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Crystallization
reagents
nitrates
crystallization
proteins
Crystallography
Proteins
organic liquids
detergents
lysozyme
Muramidase
Detergents
crystallography
Hydrogen
Hydrogen bonds
Salts
chemistry
hydrogen bonds
Ligands
salts

ASJC Scopus subject areas

  • Structural Biology

Cite this

Ethylammonium nitrate : A protein crystallization reagent. / Garlitz, Jennifer A.; Summers, Catherine A.; Flowers, Robert A.; Borgstahl, Gloria E.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 12, 01.12.1999, p. 2037-2038.

Research output: Contribution to journalArticle

Garlitz, Jennifer A. ; Summers, Catherine A. ; Flowers, Robert A. ; Borgstahl, Gloria E. / Ethylammonium nitrate : A protein crystallization reagent. In: Acta Crystallographica Section D: Biological Crystallography. 1999 ; Vol. 55, No. 12. pp. 2037-2038.
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