Ethanol promotes intestinal tumorigenesis in the MIN mouse

Hemant K. Roy, James M. Gulizia, William J. Karolski, Anne Ratashak, Michael Floyd Sorrell, Dean Tuma

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Epidemiological studies suggest that alcohol consumption increases the risk of developing colorectal cancer; however, these data are confounded by numerous cosegregating variables. Previous experimental reports with the rodent carcinogen model have also yielded discordant results. To clarify the alcohol-colon cancer relationship, we used the MIN (multiple intestinal neoplasia) mouse, a genetic model of intestinal tumorigenesis. Twenty-four MIN mice were randomized to ethanol supplementation in the drinking water (15% alternating with 20% on a daily basis) or control. Mice were sacrificed after 10 weeks, and the intestinal tumors were scored under magnification. Tissue sections were assessed for apoptosis and cell proliferation rates, along with the presence of the malondialdehyde-acetaldehyde (MAA) adduct, a mutagenic adduct associated with ethanol consumption. Ethanol supplementation resulted in a significant increase in tumor number (135 ± 35%; P = 0.027 versus control). The induction of tumorigenesis by ethanol was most dramatic in the distal small bowel (1.67 ± 56%; P = 0.01). In the uninvolved intestinal mucosa, there was no difference in proliferative or apoptotic indices. Cytoplasmic and nuclear MAA adducts were detected in both ethanol-treated and control mice. We demonstrated that ethanol ingestion increased intestinal tumorigenesis in the MIN mouse model. Furthermore, whereas mechanisms remain incompletely elucidated, our data implicate formation of MAA adducts. This report provides further support that ethanol consumption is a risk factor for colorectal cancer.

Original languageEnglish (US)
Pages (from-to)1499-1502
Number of pages4
JournalCancer Epidemiology Biomarkers and Prevention
Volume11
Issue number11
StatePublished - Nov 1 2002

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Carcinogenesis
Ethanol
Acetaldehyde
Malondialdehyde
Neoplasms
Colorectal Neoplasms
Genetic Models
Intestinal Mucosa
Drinking Water
Alcohol Drinking
Carcinogens
Colonic Neoplasms
Epidemiologic Studies
Rodentia
Eating
Alcohols
Cell Proliferation
Apoptosis

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Roy, H. K., Gulizia, J. M., Karolski, W. J., Ratashak, A., Sorrell, M. F., & Tuma, D. (2002). Ethanol promotes intestinal tumorigenesis in the MIN mouse. Cancer Epidemiology Biomarkers and Prevention, 11(11), 1499-1502.

Ethanol promotes intestinal tumorigenesis in the MIN mouse. / Roy, Hemant K.; Gulizia, James M.; Karolski, William J.; Ratashak, Anne; Sorrell, Michael Floyd; Tuma, Dean.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 11, No. 11, 01.11.2002, p. 1499-1502.

Research output: Contribution to journalArticle

Roy, HK, Gulizia, JM, Karolski, WJ, Ratashak, A, Sorrell, MF & Tuma, D 2002, 'Ethanol promotes intestinal tumorigenesis in the MIN mouse', Cancer Epidemiology Biomarkers and Prevention, vol. 11, no. 11, pp. 1499-1502.
Roy HK, Gulizia JM, Karolski WJ, Ratashak A, Sorrell MF, Tuma D. Ethanol promotes intestinal tumorigenesis in the MIN mouse. Cancer Epidemiology Biomarkers and Prevention. 2002 Nov 1;11(11):1499-1502.
Roy, Hemant K. ; Gulizia, James M. ; Karolski, William J. ; Ratashak, Anne ; Sorrell, Michael Floyd ; Tuma, Dean. / Ethanol promotes intestinal tumorigenesis in the MIN mouse. In: Cancer Epidemiology Biomarkers and Prevention. 2002 ; Vol. 11, No. 11. pp. 1499-1502.
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