Estradiol regulates AQP2 expression in the collecting duct: A novel inhibitory role for estrogen receptor α

Muhammad Umar Cheema, Debra L. Irsik, Yan Wang, William Miller-Little, Kelly A. Hyndman, Eileen S. Marks, Jørgen Frøkiær, Erika I Boesen, Rikke Norregaard

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

While there is evidence that sex hormones influence multiple systems involved in salt and water homeostasis, the question of whether sex hormones regulate aquaporin-2 (AQP2) and thus water handling by the collecting duct has been largely ignored. Accordingly, the present study investigated AQP2 expression, localization and renal water handling in intact andovariectomized (OVX) female rats, with and without estradiol or progesterone replacement. OVX resulted in a significant increase in urine osmolality and increase in p256-AQP2 in the renal cortex at 7 days post-OVX, as well as induced body weight changes. Relative to OVX alone, estradiol repletion produced a significant increase in urine output, normalized urinary osmolality and reduced both total AQP2 (protein and mRNA) and p256-AQP2 expression, whereas progesterone repletion had little effect. Direct effects of estradiol on AQP2 mRNA and protein levels were further tested in vitro using the mpkCCD rincipal cell line. Estradiol treatment of mpkCCD cells reduced AQP2 at both the mRNA and protein level in the absence of deamino-8-D-AVP (dDAVP) and significantly blunted the dDAVPinduced increase in AQP2 at the protein level only. We determined that mpkCCD and native mouse collecting ducts express both estrogen receptor (ER) a and ERß and that female mice lacking ERa displayed significant increases in AQP2 protein compared with wildtype littermates, implicating ERa in mediating the inhibitory effect of estradiol on AQP2 expression. These findings suggest that changes in estradiol levels, such as during menopause or following reproductive surgeries, may contribute to dysregulation of water homeostasis in women.

Original languageEnglish (US)
Pages (from-to)F305-F317
JournalAmerican Journal of Physiology - Renal Physiology
Volume309
Issue number4
DOIs
StatePublished - Aug 18 2015

Fingerprint

Aquaporin 2
Estrogen Receptors
Estradiol
Water
Gonadal Steroid Hormones
Proteins
Osmolar Concentration
Messenger RNA
Progesterone
Homeostasis
Urine
Kidney
Body Weight Changes
Menopause
Salts

Keywords

  • AQP2
  • ERα
  • Estrogen
  • KO mice

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Cheema, M. U., Irsik, D. L., Wang, Y., Miller-Little, W., Hyndman, K. A., Marks, E. S., ... Norregaard, R. (2015). Estradiol regulates AQP2 expression in the collecting duct: A novel inhibitory role for estrogen receptor α. American Journal of Physiology - Renal Physiology, 309(4), F305-F317. https://doi.org/10.1152/ajprenal.00685.2014

Estradiol regulates AQP2 expression in the collecting duct : A novel inhibitory role for estrogen receptor α. / Cheema, Muhammad Umar; Irsik, Debra L.; Wang, Yan; Miller-Little, William; Hyndman, Kelly A.; Marks, Eileen S.; Frøkiær, Jørgen; Boesen, Erika I; Norregaard, Rikke.

In: American Journal of Physiology - Renal Physiology, Vol. 309, No. 4, 18.08.2015, p. F305-F317.

Research output: Contribution to journalArticle

Cheema, MU, Irsik, DL, Wang, Y, Miller-Little, W, Hyndman, KA, Marks, ES, Frøkiær, J, Boesen, EI & Norregaard, R 2015, 'Estradiol regulates AQP2 expression in the collecting duct: A novel inhibitory role for estrogen receptor α', American Journal of Physiology - Renal Physiology, vol. 309, no. 4, pp. F305-F317. https://doi.org/10.1152/ajprenal.00685.2014
Cheema, Muhammad Umar ; Irsik, Debra L. ; Wang, Yan ; Miller-Little, William ; Hyndman, Kelly A. ; Marks, Eileen S. ; Frøkiær, Jørgen ; Boesen, Erika I ; Norregaard, Rikke. / Estradiol regulates AQP2 expression in the collecting duct : A novel inhibitory role for estrogen receptor α. In: American Journal of Physiology - Renal Physiology. 2015 ; Vol. 309, No. 4. pp. F305-F317.
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