Estimating the probability that a genomic library contains a specific DNA sequence

C. O. Gardner, Kent M Eskridge, H. T. Zhang, D. P. Weeks

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A genomic library generally contains a random collection of DNA fragments from one organism individually cloned into cells of a host organism using plasmid or viral vectors. For a genomic library to be reliable, it is crucial that the library have a high probability of containing a complete copy of any particular gene being studied. In this article, we compare several different methods of estimating this probability based on data from a genomic library of Chlamydomonas reinhardtii DNA. Results suggest that a method based on estimated insert size will generally be preferred because of its precision and cost effectiveness relative to other methods.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalJournal of Agricultural, Biological, and Environmental Statistics
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Genomic Library
DNA sequences
genomic libraries
DNA Sequence
Genomics
genomics
nucleotide sequences
DNA
Chlamydomonas reinhardtii
organisms
Chlamydomonas Reinhardtii
cost effectiveness
Cost effectiveness
Libraries
Cost-Benefit Analysis
Cost-effectiveness
plasmids
Plasmids
Genes
methodology

Keywords

  • Binomial proportion
  • Gene library
  • Reliability

ASJC Scopus subject areas

  • Statistics and Probability
  • Environmental Science(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Agricultural and Biological Sciences(all)
  • Statistics, Probability and Uncertainty
  • Applied Mathematics

Cite this

Estimating the probability that a genomic library contains a specific DNA sequence. / Gardner, C. O.; Eskridge, Kent M; Zhang, H. T.; Weeks, D. P.

In: Journal of Agricultural, Biological, and Environmental Statistics, Vol. 4, No. 1, 01.01.1999, p. 1-8.

Research output: Contribution to journalArticle

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