Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera: Thripidae) via Next Generation Sequencing

Aaron M. Dickey, Andrew J. Trease, Antonella Jara-Cavieres, Vivek Kumar, Matthew K. Christenson, Lakshmi Prasad Potluri, J. Kent Morgan, Robert G. Shatters, Cindy L. McKenzie, Paul H. Davis, Lance S. Osborne

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.

Original languageEnglish (US)
Pages (from-to)362-366
Number of pages5
JournalFlorida Entomologist
Volume97
Issue number2
DOIs
StatePublished - Jun 2014

Fingerprint

Scirtothrips dorsalis
Thripidae
Thysanoptera
Stenotrophomonas
insect
Propionibacterium
plant pests
disease vectors
insects
insect control
Actinobacteria
Proteobacteria
bacteria
plant diseases and disorders
invasive species
symbionts
Pseudomonas
disease vector
thrips
bacterium

Keywords

  • Metagenomics
  • Next Generation Sequencing
  • chilli thrips

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Dickey, A. M., Trease, A. J., Jara-Cavieres, A., Kumar, V., Christenson, M. K., Potluri, L. P., ... Osborne, L. S. (2014). Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera: Thripidae) via Next Generation Sequencing. Florida Entomologist, 97(2), 362-366. https://doi.org/10.1896/054.097.0204

Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera : Thripidae) via Next Generation Sequencing. / Dickey, Aaron M.; Trease, Andrew J.; Jara-Cavieres, Antonella; Kumar, Vivek; Christenson, Matthew K.; Potluri, Lakshmi Prasad; Morgan, J. Kent; Shatters, Robert G.; McKenzie, Cindy L.; Davis, Paul H.; Osborne, Lance S.

In: Florida Entomologist, Vol. 97, No. 2, 06.2014, p. 362-366.

Research output: Contribution to journalArticle

Dickey, AM, Trease, AJ, Jara-Cavieres, A, Kumar, V, Christenson, MK, Potluri, LP, Morgan, JK, Shatters, RG, McKenzie, CL, Davis, PH & Osborne, LS 2014, 'Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera: Thripidae) via Next Generation Sequencing', Florida Entomologist, vol. 97, no. 2, pp. 362-366. https://doi.org/10.1896/054.097.0204
Dickey, Aaron M. ; Trease, Andrew J. ; Jara-Cavieres, Antonella ; Kumar, Vivek ; Christenson, Matthew K. ; Potluri, Lakshmi Prasad ; Morgan, J. Kent ; Shatters, Robert G. ; McKenzie, Cindy L. ; Davis, Paul H. ; Osborne, Lance S. / Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera : Thripidae) via Next Generation Sequencing. In: Florida Entomologist. 2014 ; Vol. 97, No. 2. pp. 362-366.
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