Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

T. Miura, J. I. Sakuragi, M. Kawamura, M. Fukasawa, E. N. Moriyama, T. Gojobori, K. I. Ishikawa, J. A.A. Mingle, V. B.A. Nettey, H. Akari, M. Enami, H. Tsujimoto, M. Hayami

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridize with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2(ALT)(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIV(mac)/SIV(sm) in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

Original languageEnglish (US)
Pages (from-to)1257-1261
Number of pages5
JournalAIDS
Volume4
Issue number12
Publication statusPublished - Dec 1 1990

    Fingerprint

Keywords

  • AIDS
  • HIV
  • Lentivirus
  • Phylogenetic tree
  • Polymerase chain reaction
  • Simian immunodeficiency virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Miura, T., Sakuragi, J. I., Kawamura, M., Fukasawa, M., Moriyama, E. N., Gojobori, T., ... Hayami, M. (1990). Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup. AIDS, 4(12), 1257-1261.