Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

Tomoyuki Miura, Jun Ichi Sakuragi, Meiko Kawamura, Masashi Fukasawa, Etsuko Moriyama, Takashi Gojobori, Koh Ichi Ishikawat, Julius A A Mingle, Victor B A Nettey, Hirofumi Akari, Masayoshi Enami, Hajime Tsujimoto, Masanori Hayami

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIVmac/SIVsm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

Original languageEnglish (US)
Pages (from-to)1257-1261
Number of pages5
JournalAIDS
Volume4
Issue number12
StatePublished - Dec 1 1990

Fingerprint

Lentivirus
HIV-2
Primate Lentiviruses
Acquired Immunodeficiency Syndrome
HIV
pol Genes
Viruses
AIDS-Related Complex
Simian Immunodeficiency Virus
DNA Primers
DNA Probes
Sequence Analysis
Surveys and Questionnaires

Keywords

  • AIDS
  • HIV
  • Lentivirus
  • Phylogenetic tree
  • Polymerase chain reaction
  • Simian immunodeficiency virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Miura, T., Sakuragi, J. I., Kawamura, M., Fukasawa, M., Moriyama, E., Gojobori, T., ... Hayami, M. (1990). Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup. AIDS, 4(12), 1257-1261.

Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup. / Miura, Tomoyuki; Sakuragi, Jun Ichi; Kawamura, Meiko; Fukasawa, Masashi; Moriyama, Etsuko; Gojobori, Takashi; Ishikawat, Koh Ichi; Mingle, Julius A A; Nettey, Victor B A; Akari, Hirofumi; Enami, Masayoshi; Tsujimoto, Hajime; Hayami, Masanori.

In: AIDS, Vol. 4, No. 12, 01.12.1990, p. 1257-1261.

Research output: Contribution to journalArticle

Miura, T, Sakuragi, JI, Kawamura, M, Fukasawa, M, Moriyama, E, Gojobori, T, Ishikawat, KI, Mingle, JAA, Nettey, VBA, Akari, H, Enami, M, Tsujimoto, H & Hayami, M 1990, 'Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup', AIDS, vol. 4, no. 12, pp. 1257-1261.
Miura T, Sakuragi JI, Kawamura M, Fukasawa M, Moriyama E, Gojobori T et al. Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup. AIDS. 1990 Dec 1;4(12):1257-1261.
Miura, Tomoyuki ; Sakuragi, Jun Ichi ; Kawamura, Meiko ; Fukasawa, Masashi ; Moriyama, Etsuko ; Gojobori, Takashi ; Ishikawat, Koh Ichi ; Mingle, Julius A A ; Nettey, Victor B A ; Akari, Hirofumi ; Enami, Masayoshi ; Tsujimoto, Hajime ; Hayami, Masanori. / Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup. In: AIDS. 1990 ; Vol. 4, No. 12. pp. 1257-1261.
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