Essential role for dnmt1 in the prevention and maintenance of MYC-Induced T-Cell lmphomas

Staci L. Peters, Ryan A. Hlady, Jana Opavska, David L Klinkebiel, Slavomira Novakova, Lynette M Smith, Robert E Lewis, Adam R Karpf, Melanie A. Simpson, Lizhao Wu, Rene Opavsky

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

DNA cytosine methylation is an epigenetic modification involved in the transcriptional repression of genes controlling a variety of physiological processes, including hematopoiesis. DNA methyltransferase 1 (Dnmt1) is a key enzyme involved in the somatici nheritance of DNA methylation and thus plays a critical role in epigenomic stability. Aberrant methylation contributes to the pathogenesis of human cancer and of hematologic malignancies in particular. To gain deeper insight into the function of Dnmt1 in lymphoid malignancies, we genetically inactivated Dnmt1 in a mouse model of MYC-induced T-cell lymphomagenesis. We show that loss of Dnmt1 delays lymphomagenesis by suppressing normal hematopoiesis and impairing tumor cell proliferation. Acute inactivation of Dnmt1 in primary lymphoma cells rapidly induced apoptosis, indicating that Dnmt1 is required to sustain T-cell lymphomas. Using high-resolution genome-wide profiling, we identified differentially methylated regions between control and Dnmt1-deficient lymphomas, demonstrating a locus-specific function for Dnmt1 in both maintenance and de novo promoter methylation. Dnmt1 activity is independent of the presence of Dnmt3a or Dnmt3b in de novo promoter methylation of the H2-Ab1 gene. Collectively, these data show for the first time that Dnmt1 is critical for the prevention and maintenance of T-cell lymphomas and contributes to aberrant methylation by both de novo and maintenance methylation.

Original languageEnglish (US)
Pages (from-to)4321-4333
Number of pages13
JournalMolecular and cellular biology
Volume33
Issue number21
DOIs
StatePublished - Oct 25 2013

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Methyltransferases
Maintenance
T-Lymphocytes
DNA
Methylation
T-Cell Lymphoma
Hematopoiesis
DNA Methylation
Epigenomics
Lymphoma
Physiological Phenomena
Neoplasms
Cytosine
Hematologic Neoplasms
Genes
Cell Proliferation
Genome
Apoptosis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Essential role for dnmt1 in the prevention and maintenance of MYC-Induced T-Cell lmphomas. / Peters, Staci L.; Hlady, Ryan A.; Opavska, Jana; Klinkebiel, David L; Novakova, Slavomira; Smith, Lynette M; Lewis, Robert E; Karpf, Adam R; Simpson, Melanie A.; Wu, Lizhao; Opavsky, Rene.

In: Molecular and cellular biology, Vol. 33, No. 21, 25.10.2013, p. 4321-4333.

Research output: Contribution to journalArticle

Peters, Staci L. ; Hlady, Ryan A. ; Opavska, Jana ; Klinkebiel, David L ; Novakova, Slavomira ; Smith, Lynette M ; Lewis, Robert E ; Karpf, Adam R ; Simpson, Melanie A. ; Wu, Lizhao ; Opavsky, Rene. / Essential role for dnmt1 in the prevention and maintenance of MYC-Induced T-Cell lmphomas. In: Molecular and cellular biology. 2013 ; Vol. 33, No. 21. pp. 4321-4333.
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