Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection

Lin Xu Wang, Michael Mellon, Dane Bowder, Meghan Quinn, Danielle Shea, Charles Wood, Shi Hua Xiang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to prevent HIV-1 infection. The VRC01 antibody mimics CD4-binding to gp120 and has broadly neutralization activities against HIV-1. We have designed a construct that can express the fusion peptide of the scFv-VRC01 antibody together with the autotransporter β-barrel domain of IgAP gene from Neisseria gonorrhoeae, which enabled surface display of the antibody molecule. Our results indicate that the scFv-VRC01 antibody molecule was displayed on the surface of the bacteria as demonstrated by flow cytometry and immunofluorescence microscopy. The engineered bacteria can capture HIV-1 particles via surface-binding and inhibit HIV-1 infection in cell culture.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalVirology
Volume475
DOIs
StatePublished - Jan 5 2015

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Keywords

  • Autotransporter
  • E. coli K12 UT5600
  • HIV-1
  • ScFv-VRC01
  • Surface display
  • β-Barrel domain

ASJC Scopus subject areas

  • Virology

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